摘要
改进了冬虫夏草药材中腺苷的含量测定方法。采用WelehromC18色谱柱（4．6mm×250mm，5μm）；以0．1％磷酸溶液（调pH2．8）-甲醇（95：5）为流动相；流速：1．0mL／min；柱温：25℃；检测波长：260nm。结果表明：腺苷在0．08432～0．42160μg（r=0．9998）范围内呈良好的线性关系；腺苷回收率为97．57％（RSD=0．83％）。本法简便、准确、重现性好，可用于冬虫夏草的含量测定。
To improve the method for the determination of adenosine in Aweto by RP-HPLC, the samples were separated on Welchrom ClS column （4. 6 min×250 mm, 5 μm） with methanol and 0. 1% phosphoric acid at a flow rate of 1.0 mL/min. The detections were done at 260 nm and 25 ℃. The results showed that adenosine presented a good linear correlation in range of 0. 08432 - 0. 42160 μg （ r = 0. 9998 ）. The average recoveries was 97.57% （ RSD = 0.83% ）. This method was simple, precious and reproducible, could be used to determine adenosine in Aweto.
出处
《广州化工》
CAS
2015年第16期149-150,225,共3页
GuangZhou Chemical Industry
关键词
冬虫夏草
腺苷
含量测定
Aweto
adenosine
geniposide
determination
