摘要
本研究采用相似生境分离方法分离魔芋内生细菌后经室内平板初筛、温室复筛试验筛选获得对魔芋软腐病具有良好防治效果的生防菌。研究共分离出127株内生细菌,经过多重筛选后获得3株抑菌效果较好的生防菌(M3,M2,1-7)。经培养性状、菌落形态观察、生理生化特性分析(Biolog系统)和16S r DNA序列分析等方法,将M3,M2,1-7分别鉴定为:枯草芽孢杆菌(Bacillus subtilis)、解淀粉芽孢杆菌(Bacillus amyloliquefaciens)和成团泛生菌(Pantoea agglomerans)。这是将寄主植物内生细菌作为生防菌防治该病害的首次报道。
In this study, 127 endophytic bacteria of konjac were isolated by similar habitat sepa- ration method. Three strains (M3, M2, 1-7 ) were screened out for control the soft rot of Amor- phophallus konjac by plate screening test and greenhouse rescreening test. The bacteria strains M3, M2, 1-7 were identified as Bacilus subtilis, Bacillus amyloliquefaciens and Pantoea agglom- erans respectively, according to the characters of cultural colonies, morphological observation, biochemical and physiological identification (Biolog carbon source utilization analysis) , 16S rDNA sequences analysis. This is the first report of use host plant endophytic bacteria as biocontrol a- gents to control the disease.
出处
《云南农业大学学报(自然科学版)》
CAS
CSCD
北大核心
2015年第4期547-553,共7页
Journal of Yunnan Agricultural University:Natural Science
基金
农业部公益性行业专项(201303015)
国家自然基金(31360002,31460458)
关键词
魔芋内生细菌
魔芋细菌性软腐病
生物防治
鉴定
endophytic bacteria
Pectobacterium carotovora subsp, carotovora
Amorphophallus konjac
biological control
identification