miR-139在结直肠癌组织中的表达及其对癌细胞生长的影响

Expression of miR-139 and its role in colorectal cancer cells

目的探讨miR-139在结直肠癌组织中的表达以及上调miR-139表达对结直肠癌细胞生长的影响。方法应用实时定量PCR法检测miR-139在20例大肠癌组织及癌旁组织中的表达,HT29细胞转染miR-139 mimics后,通过MTT、Softagar方法检测miR-139对大肠癌细胞增殖的影响;Transwell实验检测miR-139对大肠癌细胞迁移和侵袭的影响。结果 miR-139在结直肠癌组织中表达量明显低于癌旁组织(P<0.01)。转染miR-139 mimics的HT29 MTT实验中第1天至第5天的吸光度(OD)值与对照组相比显著减低。Softagar实验中计数对照组每个视野(41±5),miR-139每个视野(72±5),差异有统计学意义(P<0.01)。Transwell小室实验显示,与阴性对照相比,转染miR-139 mimics后,HT29细胞的迁移及侵袭能力明显下降。结论 miR-139在结直肠癌组织中低表达,miR-139表达上调可抑制HT29生长、迁移及侵袭能力,miR-139有潜在抑癌作用。

Objective To explore the expression of miR-139 in colorectal cancer and study its role in colorectal cancer HT29 cells in vitro. Methods Real-Time PCR was used to analyze the expression of miR-139 in 20 color- ectal cancer tissues and their matched adjacent tissues. HT29 cells were transfeeted with a miR-139 mimics, then MTT and Soflagar methods were used to detect the proliferation of the cells. The migration and invasion of HT29 cells were investigated using Transwell assay. Results The expression of miR-139 was down-regulated in colorectal cancer tissues compared with those in adjacent tissues （P 〈0. 01 ）. According to MTT method, from the first day to fifth day,the OD of HT29 cells transfected with miR-139 mimics significantly reduced compared with the control group. According to Softagar method, the control group counts（41± 5 ）each field compared with miR-139 （72 ± 5 ） each field （ P 〈 0. 01 ）. According to Transwell assay, compared with the control group, transfection of miR-139 mimics inhibited the migration and invasion of HT29 cells. Conclusion The expression level of miR-139 is lower in colorectal cancer. Transfection of HT29 cells with a miR-139 mimics can inhibit the cell proliferation,migration and invasion in vitro, suggesting that miR-139 is a potent tumor suppressor.