左归丸改善惊恐伤肾脑缺血大鼠记忆力的实验研究

Experimental study of Zuogui Wan on improvement memory in cerebral ischemia model rats of kidney deficiency induced by panic

目的观察惊恐伤肾对大鼠记忆力的影响及左归丸的脑保护作用。方法将40只雌性Waster大鼠按体重随机分为4组,分别为假手术组、脑缺血模型组、肾虚模型组与实验组,后3组进行大脑中动脉阻塞(MCAO)造模手术;肾虚模型组于MCAO手术前10天,用"猫吓鼠"法进行恐吓,实验组于MCAO手术前3天,灌胃1.62 g·kg-1左归丸汤剂2 m L,每日1次,连续给药10 d,其他3组给予等体积生理盐水。新奇物认知法检测大鼠记忆力;免疫组化法及免疫印迹法检测脑源性神经营养因子(BDNF)的表达。结果与假手术组比较,脑缺血模型组大鼠记忆力下降;与脑缺血模型组比较,肾虚模型组大鼠记忆力下降且BDNF表达明显下调(P<0.05)。与肾虚模型组比较,实验组大鼠记忆力明显改善,同时BDNF表达量明显增加(P<0.05)。结论惊恐伤肾可加重大鼠缺血后记忆障碍,左归丸可以改善惊恐伤肾大鼠缺血后记忆力,这种作用有可能通过上调BDNF来实现。

Objective To observe the effects of Zuogui Wan on the memory of the rats with panic injury and the neuroprotective effect.Methods Forty female Waster rats were randomly divided into 4groups： sham group,model- brain group,model- kidney group,test group. Middle cerebral artery occlusion（ MCAO） was operated in rats except sham group,and ischemia- reperfusion were executed 2 hours after MCAO. The model- kidney group was threatened by the cat in 10 days before MCAO operation. The rats in the test group orally 1. 62 g·kg^- 1Zuogui Wan Decoction 2 mL at the time of 3 days before MCAO operation,1 times a day continuously for 10 days. The other 3 groups were given equal volume of saline. The memory in each group rats was detected by novel object recognition task. The neuron injury in rats was observed by HE staining. The expression of brain- derived neurotrophic factor（ BDNF） was assessed by immunohistochemistry and Western blot. Results Compared with sham group,memory decline after MCAO in model- brain group rats. Compared with the model- brain group,memory impairment was aggravated induced by ischemia and downregulated of expression of BDNF in model- kidney group,which was statistically difference（ P〈0. 05）. Compared with the model- kidney group memory in rats were significantly improved and the expression of BDNFwas increased in the test group,which was statistically difference（ P〈0. 05）. Conclusion Kidney deficiency induced by panic can significant aggravate memory impairment after cerebral ischemic injury. Zuogui Wan can improve memory ability of rats of kidney deficiency induced by panic after ischemia,which is likely achieved by upregulation of the expression of BDNF.