基于GC-MS的植物代谢组学研究

GC-MS-based plant metabolomics researches

代谢组学是系统生物学的重要组成部分。在众多代谢组学分析技术中,气相色谱-质谱联用(GC-MS)技术较为成熟,分辨率高、灵敏度高、重现性好,拥有大量标准质谱图数据库且成本相对低廉,很早就应用到植物代谢组学研究领域,迄今仍然是主要分析平台之一。现从GC-MS技术的核心原理、衍生化方法以及数据采集、分析和数据库等方面介绍了GC-MS植物代谢组学分析技术。同时,综述了该技术在基因功能研究、植物代谢遗传机理、植物抗逆、生物技术和生物育种中的应用。

Metabolomics is one of the very important parts of systems biology. Gas chromatography-mass spectrometry （GC-MS） as a mature technology has a lot of advantages, such as high resolution, high sensitivity, good reproducibility, with a large standard library and relatively low cost. It has been early applied to the field of plant metabolomics, and now still is the main technical platform for plant metabolomics. This review introduces the technology from the aspects of basic mechanism, derivatization methods, data acquisition, data analysis and database. Meanwhile, the review comprehensively summarizes the applications of GC-MS-based plant metabolomics to the analysis of gene function, the genetic mechanisms of plant metabolism, plant metabolic response to stress and applications to biotechnology and bio-breeding.