桃核承气汤联合奥曲肽防治大鼠重症急性胰腺炎并发腹腔高压

Taohe Chengqi decoction combined with octreotide for prevention and treatment of intra-abdominal hypertension in rats with severe acute pancreatitis

目的:观察中药桃核承气汤与奥曲肽合用对重症急性胰腺炎(severe acute pancreatitis,SAP)并发腹腔高压(intra-abdominal hypertension,IAH)大鼠血清肿瘤坏死因子-α(tumor necrosis factorα,TNF-α)、白介素-6(interleukin-6,IL-6)含量及腹腔压的影响.方法:SD大鼠90只随机分为正常组(18只)、模型组(18只)、中药组(18只)、奥曲肽组(18只)、中药联合奥曲肽组(18只).各组分别于造模成功后3、6 h,中药组经胃管灌服桃核承气汤(2.0 mL/100 g);奥曲肽组于大鼠后肢皮下注射奥曲肽(0.001 mg/kg);中药和奥曲肽联合应用组,于大鼠后肢根部皮下注射奥曲肽(0.001 mg/kg)及经胃管灌服桃核承气汤(2.0 mL/100 g).各组造模成功后6、12、18 h,测定血清TNF-α、IL-6含量和相应时间点腹腔压.结果:模型组大鼠血清TNF-α、IL-6水平及腹腔压明显升高(P<0.05).中药组、奥曲肽组、中药联合奥曲肽组与模型组比较,大鼠血清TNF-α、IL-6水平、腹腔压有不同程度下降.其中中药联合奥曲肽组下降较为明显(P<0.05).结论:中药和奥曲肽联合应用可显著降低SAP大鼠血清TNF-α、IL-6水平,降低大鼠腹腔压.

AIM： To investigate the influence of Taohe Chengqi decoction alone or combined with octreotide on serum contents of tumor necrosis factor α （TNF-α） and interleukin-6 （IL-6） and intra-abdominal pressure in rats with severe acute pancreatitis （SAP）. METHODS： Ninety SD rats were randomly divided into a control group （n = 18）, a model group （n = 18）, a Chinese medicine group （n = 18）, an octreotide group （n = 18） and a Chinese medicine combined with octreotide group （n = 18）. At 3 and 6 h after SAP was induced successfully, the rats were administered orally with Taohe Chengqi decoction （2.0 mL/100 g）, or injected with octreotide （0.001 mg/kg）, or both. Serum contents of TNF-α and IL-6 and intra-abdominal pressure were measured 6, 12, and 18 h after operation. RESULTS： The contents of TNF-α and IL-6 in serum and intra-abdominal pressure in the model group were significantly increased （P 〈 0.05）. Compared with the model group, the contents of TNF-α and IL-6 in serum and intra-abdominal pressure were reduced to varying degrees inthe Chinese medicine group, octreotide group and Chinese medicine combined with octreotide group, with the changes being most prominent in the Chinese medicine combined with octreotide group （P 〈 0.05）. CONCLUSION： The contents of TNF-α and IL-6 in serum and intra-abdominal pressure significantly decrease in SAP rats. Taohe Chengqi decoction combined with octreotide can alleviate pathological injury in SAP rats and delay the course of SAP.