海口梅毒螺旋体分子流行病学研究

Molecular epidemiological research of Treponema palliduminfection in Haikou

目的:探讨海口市梅毒螺旋体基因型分布及其分子流行病学特征。方法:收集102例疑似梅毒硬下疳的生殖器溃疡标本,采用暗视野镜检和PCR方法检测梅毒螺旋体。对PCR检测结果阳性标本采用巢式PCR扩增梅毒螺旋体的酸性重复蛋白基因(arp)和苍白螺旋体重复基因族(tpr),琼脂糖凝胶电泳分析arp基因重复序列数和tpr基因酶切片段多态性。结果:共检测102例疑似硬下疳,暗视野镜检发现55例(53.7%)有梅毒螺旋体;梅毒螺旋体bmpPCR检测结果89例阳性,占87.3%。72例Tp基因分型发现有9个基因型,分子亚型分布依次为14d(28例,占38.9%),12d(12例,占16.7%),13d(11例,占15.3%),14b(9例,占12.5%),14a(5例,占6.9%),12a(3例,占4.2%),15d(2例,占2.8%),10d(1例,占1.4.%),12i(1例,占1.4.%)。结论:海口市地区梅毒螺旋体基因分型以14d为优势流行株,梅毒螺旋体bmpPCR检测有较高的敏感性。

Objective：To discuss genotypes distribution and molecular epidemiological characters of Treponema pallidum infection in Haikou.Methods：A total of 102 specimens genital ulcers were collected from patients with suspected syphilitic chancre admitted during March 2012-February 2013,which were detected by dark-field microscope and PCR method.Acidic repeat protein（arp）gene of Treponema pallidum and Treponema pallidumrepeat（tpr）gene families were amplified in PCR-positive samples by nested PCR.The number of repeats presented in the arp gene and the restriction fragment length polymorphism by MseI in the tpr gene were analyzed by electrophoresis.Results：Out of 102 patients with suspected chancre,55cases（53.2%）were positive by dark-field microscopy and 89cases（87.3%）by Treponema pallidum bmp PCR.Seven genotypes were found in 72 cases,including 14d（28,38.9%）,12d（12,16.7%）,13d（11,15.3%）,14b（9,12.5%）,14a（5,6.9%）,12a（3,4.2%）,15d（2,2.8%）10d（1,1.4.%）,12i（1,1.4%）.Conclusions：Genotype 14 dis the predominant type of Treponema pallidumin Haikou and bmp PCR has high sensitivity.