复发性口腔溃疡患者血清中TNF-α、IL-2,6与免疫功能的相关性

Correlation of TNF-α,IL-2,6 and immune function with recurrent oral ulcers

目的：探讨复发性口腔溃疡（ROU）患者血清中肿瘤坏死因子α（TNF-α）、白细胞介素-2,6（IL-2,6）与免疫功能的相关性。方法：2014年1月-2014年12月选取口腔科就诊的68例复发性口腔溃疡患者为研究对象（ROU组）,另选取同期行身体检查的60例健康体检者为对照组,采用ELISA测定两组血清及龈沟液中TNF-α,IL-2,IL-6水平,采用流式细胞术测定两组免疫细胞（CD3^＋、CD4^＋、CD4^＋/CD8^＋）水平。采用Pearson相关因素分析TNF-α,IL-2,IL-6与免疫细胞的关系。结果：ROU组患者血清中TNF-α、IL-2,IL-6水平显著高于对照组（P〈0.05）。ROU组CD4^＋、CD4^＋/CD8^＋水平显著低于对照组,而CD8^＋水平高于对照组（P〈0.05）。经Pearson相关因素分析可知,血清中TNF-α与IL-2,IL-6及CD4^＋/CD8^＋呈正相关（P〈0.05）,而与CD3^＋、CD4^＋无相关性（P〉0.05）。结论：复发行口腔溃疡的发生可能与患者免疫功能紊乱有关,通过测定TNF-α可反映免疫功能状态,有利于判断预后。

Objective： To investigate the correlation of TNF-a, IL-2,6 and immune function with patients of recurrent oral ulcers（ROU）. Methods： The levels of TNF-α, IL-2,6 of 68 patients with recurrent oral ulcers and 60 cases of healthy control group were detected with ELISA assay from January 2014 to December 2014. The levels of immune cells （CD3^＋ , CD4^＋ , CD4^＋/CD8^＋ ） of two groups were detected with flow cytometry. The relationship of TNF-α, IL-2, IL-6 and immune function was detected with immune cells. Results： The levels of TNF-α, IL-2, IL-6 of ROU group were higher than control groups （P〈0.05）. The levels of CD4^＋ , CD4^＋/CD8^＋ of ROU group were lower than the control group, while the levels of CD8^＋ were higher than control groups （P〈0.05）. The levels of TNF-α were positively correlated with IL-2, IL-6 and CD4^＋/CD8^＋ （P（ 0.05）, but was of no correlation with the CD3^＋ , CD4^＋ （P〉 0.05）. Conclusion： The Re-occurrence of oral ulcers may be associated with immune dysfunction in patients with relevant. TNF-α may reflect immune function, and is helpful in the prognosis.