摘要
目的研究核转录因子-κB(NF-κB)特异性抑制剂吡咯烷二硫代氨基甲盐酸(PDTC)对人鼻咽癌CNE-2Z细胞生长增殖及其细胞核抗原(PCNA)的影响。方法不同浓度的PDTC(25、50、100μmol/L)作用CNE-2Z细胞不同时间后(24、48、72 h),四甲基偶氮唑蓝(MTT)比色法检测PDTC对CNE-2Z细胞增殖的抑制效应;不同浓度的PDTC(25、50、100μmol/L)作用于CNE-2Z细胞48 h后,流式细胞术(FCM)分析细胞周期,免疫组化(SP)法检测不同强度PDTC作用下CNE-2Z细胞增殖细胞核抗原(PCNA)的表达情况。结果 25、50、100μmol/L PDTC处理的CNE-2Z细胞增殖活性明显受到抑制(P<0.05),并呈时间和剂量依赖;流式细胞术结果表明S期细胞数减少,细胞受阻于G0/G1期。与对照(无PDTC处理)比较,PDTC作用后CNE-2Z细胞PCNA表达降低(P<0.05),并呈明显的剂量依赖。结论 PDTC具有显著抑制人鼻咽癌CNE-2Z细胞生长的作用,是一种有潜力的抑制鼻咽癌细胞增殖的药物。
Objective To explore the effects of pyrrolidine dithiocarbamate （PDTC）,a specific inhibitor of NF-κB on the proliferation of human nasopharyngeal carcinoma cell line CNE-2Z and its effect on proliferation cell nuclear antigen （PCNA） expression. Methods CNE-2Z cells were respectively treated with different concentration （25, 50, 100 Ixmol/L） PDTC and different time （24, 48, 72 h）. The inhibition effects of cell proliferation were assayed by four methyl thiazolyl tetrazolium colorimetric（MTY） method. Cell cycle was analyzed by flow cytometry and the expression of proliferating cell nuclear antigen （PCNA） was detected by immunocytochemical method after treating CNE-2Z cells with different concentration PDTC （25, 50, 100 μmol/L） after 48 h. Results The proliferation activity of CNE-2Z cells dealed with 25, 50, 100 μmol/L PDTC was inhibited obviously （P 〈 0.05）, and it showed a time-dependent and dose-dependent manner. FCM result showed that the cell population reduced in S phase and the cell cycle was arrested in G0/G1 phase. Compared with the control （no PDTC）, the expression of PCNA of CNE-2Z cells was down regulated after PDTC dealed with （P 〈 0.05）, and it showed a dose-dependent manner. Conclusion The growth of CNE-2Z can be inhibited and the expression of PCNA in CNE-2Z can could be down-regulated by PDTC. PDTC may be a potential chemotherapeutics for the proliferation of nasopharyngeal carcinoma cell line.
出处
《中国医药导报》
CAS
2015年第25期11-14,19,F0004,共6页
China Medical Herald
基金
湖北省宜昌市医疗卫生科研项目(AL4301-16)
