马齿苋水提物对阿尔茨海默病模型小鼠学习行为的干预作用

Intervention Effects of Water Extracts from Portulacu Oleracea L. on Learning Behavior of Alzheimer Model Mice

目的观察马齿苋水提物对AlCl3诱发阿尔茨海默病(Alzheimer’s disease,AD)模型小鼠学习行为及脑组织中超氧化物歧化酶(SOD)、乙酰胆碱酯酶(Acetyl cholinesterase,Ach E)活性和丙二醛(MDA)浓度的影响。方法小鼠随机分为2批6组,正常组、模型组、吡拉西坦(3.0 g·kg-1)组、马齿苋水提物(2.6,1.3,0.65 g·kg-1)组。正常组口服生理盐水,Al Cl3200 mg·kg-1给药制备AD小鼠模型。连续造模和口服给药40 d。使用避暗仪和水迷宫仪,检测马齿苋水提物对AD模型小鼠的学习行为的变化,测定脑组织中MDA含量、SOD和Ach E活性。结果不同浓度马齿苋水提物明显延长小鼠避暗潜伏期,减少错误次数;缩短小鼠水迷宫到达时间和减少错误次数;明显升高脑组织SOD活性,降低AchE活性和MDA含量。结论马齿苋对AD模型小鼠学习行为具有明显的改善作用,其作用可能与升高SOD活性,降低MDA含量和AchE活性有关。

OBJECTIVE To observed effects of water extract from Portulacu oleracea L. of learning behavior and SOD, Acetyl cholinesterase（AchE） activities, MDA content in brain tissue of Alzheimer＇s disease（AD） mice are caused by A1C13. METHODS The mice 6 of groups of 2 batches： control group, model group, piracetam（3.0 g.kg-1） group and 3 groups of water extract from Portulacu oleracea L. （2.6, 1.3, 0.65 g.kg-1）. Normal control group PO saline. Copied AD mice models by PO A1C13 200 mg.kg-l for 40 d. Observed the learning memory improvement of passive avoidance with darkness avoidance test and water maze test, and determined contents of MDA, activities of SOD and AchE in brain tissues of mice. RESULTS The different concentration water extract from Portulacu oleracea L. could significantly prolong latency and reduce for error frequency of mice, it could obviously short times of arriving water maze and reduce for error frequency of mice than model group. It could substantially improve SOD activity, reduce AchE contents and activity MDA in brain tissues of mice. CONCLUSION The water extracts from Portulacu oleracea L. have obvious improvement effects of learning memory in AD model mice, its mechanism may be related to improves SOD activity, to reduce MDA content and AchE activity.