HPLC同时测定黄石感冒片中阿魏酸、芦丁、大黄酸、大黄素和大黄酚的含量

Simultaneous Determination of Ferulic Acid, Rutin, Rhein, Emodin and Chrysophanol in Huangshiganmao Tablets by HPLC

目的建立HPLC同时测定黄石感冒片中阿魏酸、芦丁、大黄酸、大黄素和大黄酚的含量。方法采用HPLC,色谱柱为Welch Topsil-C18柱（4.6 mm×250 mm,4μm）;以甲醇-0.1%磷酸溶液（78∶22）为流动相;流速：1.0 m L·min-1;柱温：30℃;检测波长：280 nm。结果阿魏酸、芦丁、大黄酸、大黄素和大黄酚峰线性范围分别为0.020 82~0.416 3μg·m L-1（r=0.999 7）,0.011 32~0.278 3μg·m L-1（r=0.999 3）,0.017 22~0.344 3μg·m L-1（r=0.999 5）,0.015 79~0.315 8μg·m L-1（r=0.999 6）和0.051 34~1.027μg·m L-1（r=0.999 9）,平均加样回收率分别为97.4%（RSD=1.1%）,95.0%（RSD=0.88%）,97.5%（RSD=1.3%）,97.4%（RSD=1.4%）和96.3%（RSD=0.87%）。结论新建方法简便、准确,可用于黄石感冒片的定量分析方法。

OBJECTIVE To establish an HPLC method for the simultaneous determination of ferulic acid, rutin, rhein, emodin and chrysophanol in Huangshiganmao tablets. METHODS A Welch Topsil-C18（4.6 mm×250 mm, 4 μm）column was used with the mobile phase of methanol-0.1% phosphate solution（78∶22） at a flow rate of 1.0 m L·min-1, the column temperature of 30 ℃ and the detection wavelength was 280 nm. RESULTS The calibration curves of ferulic acid, rutin, rhein, emodin and chrysophanol were linear over the ranges of 0.020 82-0.416 3 μg·m L-1（r=0.999 7）, 0.011 32-0.278 3 μg·m L-1（r=0.999 3）, 0.017 22-0.344 3 μg·m L-1（r=0.999 5）, 0.015 79-0.315 8 μg·m L-1（r=0.999 6） and 0.051 34-1.027 μg·m L-1（r= 0.999 9） respectively, and the average recoveries were 97.4%（RSD=1.1%）, 95.0%（RSD=0.88%）, 97.5%（RSD=1.3%）, 97.4%（RSD=1.4%） and 96.3%（RSD=0.87%） respectively. CONCLUSION This method was simple, accurate and can be used for quantitative analysis method of Huangshiganmao tablets.