羧甲基壳聚糖锌亚急性毒性研究

Subacute Toxicity of Carboxymethyl Chitosan-zinc

为评价羧甲基壳聚糖锌的亚急性毒性,30只雌雄各半的昆明种小鼠随机分为实验组1、实验组2和对照组,以两种不同锌离子络合量的羧甲基壳聚糖锌为材料,制取生理盐水浸提液,对实验组1和实验组2小鼠腹腔注射浸提液28 d,测定体重、相关血液生化指标、相关脏器指数,并进行各器官病理学检查。结果表明实验过程中各组小鼠基本生理状况均表现正常,实验结束后实验组1雄性小鼠血清ALP和UA含量均低于对照组,呈显著性差异(P<0.05),实验组2雄性小鼠血清UA含量低于对照组,呈显著性差异(P<0.05),其它血清生化指标与对照组比较无显著性差异。实验组2雌性小鼠肾脏指数高于对照组,差异显著(P<0.05),两实验组小鼠其它脏器指数均正常,与对照组比较无显著性差异。各组雌雄小鼠心、肝、脾、肺、肾以及胸腺外观观察与病理切片观察均未见明显病变,表明两种羧甲基壳聚糖锌对小鼠未表现明显的毒副作用。

In order to evaluate the subacute toxicity of carboxymethyl chitosan-zinc, thirty Kunming mice,half male and half female,were divided into three groups：experimental group 1,experimental group 2 and control group.The saline maceration extract was made from two kinds of carboxymethyl chitosan-zinc complexes with different content of zinc ions.Mice of experimental groups were given maceration extract by intraperitoneal injection for 28 days,while control group mice were given the same daily amount of normal saline.After that,the weight,related blood biochemical indexes and viscera indexes of mice were measured and pathological examination of organs was done.The results showed that the basic physiological state of all mice was normal in the experiment,but at the end of experiment,the serum content of ALP and UA in the male mice of experimental group 1 was significant lower than that of control group （P〈0.05）;the serum content of UA in the male mice of experimental group 2 were significant lower than that of control group （P〈0.05）;no significant statistical difference was detected on other serum biochemical indexes between experimental groups and control group;the renal index of female mice in experimental group 2 was significant higher than that of control group （P〈0.05）,while other visceral indexes of experimental groups mice were normal,coupled with no significant statistical difference between experimental groups and control group;through the visual observation and pathological observation of heart,liver,spleen,lung,kidney and thymus,no obvious lesions were found,showing that the two kinds of carboxymethyl chitosan-zinc complexes had no evident toxic effects on mice.