Folin-Ciocalteu比色法测定铜藻中褐藻多酚含量的研究

Determination of Phlorotannins in Sargassum horneri Using Folin-Ciocalteu Colorimetric Method

以没食子酸为标准品，使用福林酚（Folin-Ciocalteu）试剂，通过紫外-可见分光光度法，对铜藻中褐藻多酚的含量进行了测定。结果表明，测定波长为760 nm，显色试剂Folin-Ciocalteu的用量为1.25 mL，0.1 g/mL Na2CO3溶液用量为4.0 mL，定容至10 mL，显色温度为30 ℃，反应时间为60 min，多酚含量在15～50 μg/mL范围内与吸光度呈良好线性关系，回归方程为Y = 0.0138X＋0.0267，R^2=0.9993。本测定方法的平均回收率为96.18%，相对标准偏差（RSD）为2.44%。该方法的稳定性、重现性、精密度以及加标回收率的相对标准偏差均符合要求，可用于铜藻中褐藻多酚含量的测定。按照本方法测定铜藻中褐藻多酚的平均含量为17.36 mg/g（RSD = 1.25 %）。

With gallic acid as the standard,the content of phlorotannins in Sargassum horneri was determined by UV-visible spectrophotometric method using Folinphenol （Folin-Ciocalteu）.The detection wavelength was set at 760 nm.The color reaction was carried out with 1.25 mL of Folin-Ciocalteu reagent and 4.0 mL of 0.1 g/mL aqueous Na2CO3 solution in darkness at 30 ℃ for 60 min.The linear range of the established standard curve was 15-50 μg/mL and the regression equation was Y=0.0138X＋0.0267,R^2=0.9993.The average recovery rate of the added sample was 96.18%,with relative standard deviation （RSD） of 2.44%.The RSDs of stability,reproducibility,precision and recovery rate of the developed method were all satisfactory.Thus,this method can be applied to determine phlorotannins of S.horneri. The average content of phlorotannins in S.horneri was 17.36 mg/g （RSD=1.25%）.