大孔树脂纯化瓜馥木总黄酮工艺及抗抑郁活性研究

Purification and Antidepression Activity of Total Flavonoids from Fissistigma oldhamii

本文采用大孔树脂纯化瓜馥木总黄酮,并评价其抗抑郁活性。以大孔树脂AB-8、HPD-600、HPD-826、X-5和D4006对瓜馥木总黄酮的吸附率和解吸率为指标筛选树脂种类,并对优选树脂的吸附特性和各影响因素进行研究,优化工艺条件。利用小鼠强迫游泳、小鼠悬尾和小鼠开野实验对瓜馥木总黄酮的抗抑郁活性进行了评价。结果表明AB-8具有较好的吸附率和解吸率,最佳纯化工艺为:上样流速为1.0 m L/min,树脂床用7 BV 5%乙醇除杂,再用8 BV 50%乙醇洗脱,洗脱流速1.0 m L/min,经AB-8大孔树脂纯化1次后,瓜馥木总黄酮含量为57.3%。瓜馥木总黄酮100 mg/kg剂量给药组能明显缩短小鼠强迫游泳和悬尾的不动时间,且该给药组小鼠的自主运动情况与阳性对照和空白之间没有显著差异。AB-8大孔树脂能较好地用于瓜馥木总黄酮的纯化,瓜馥木总黄酮具有明确的抗抑郁活性。

To study the purification technology of total flavonoids from Fissistigma oldhamii by macroporous adsorptive resins and evaluate its antidepression activity.The adsorption and desorption capacity of 5 resins （AB-8,HPD-600,HPD-826,X-5 and D4006） for total flavonoids was investigated to select the optimal macroporous resin.The adsorption characters and the influencing factors of the selected macroporous resin were also investigated to optimize the purification technology.The classical animal models of depression including the forced swimming of the mouse,tail suspension and open-field test were applied to evaluate the antidepressant-like effect of total flavonoids from F.oldhamii. The results demonstrated that AB-8 macroporous resin had the best property for the purification of total flavonoids.The optimized conditions were as follows：the flow rate was set at 1.0 mL/min,the elution reagent used was 50% ethanol,and the volume of elution was 8 BV.Under the optimized purification conditions,the content of total flavonoids was 57.3%.The result showed that administration at 100 mg/kg significantly reduced immobility time in TST （being P〈0.01,P〈0.05,respectively）,and in FST （being P〈0.01,P〈0.05,respectively）,but did not cause significant changes in crossing numbers compared to the vehicle group.AB-8 macroporous resin was suitable to refine total flavonoids from F.oldhamii. Total flavonoids from F.oldhamii showed a clear antidepression activity in different animal models of depression.