榅桲总多酚的纯化工艺及PTP1B抑制作用研究

Purification and PTP1B Inhibition Activity of Total Polyphenols from Cydonia oblonga Mill.

通过采用11种大孔吸附树脂对榅桲多酚粗提物的静态吸附和解吸试验,筛选出适合分离纯化榅桲总多酚的最优树脂,并对其动态吸附特性和影响因素进行研究。结果表明:最佳纯化工艺条件为:AB-8大孔吸附树脂做吸附填料,上样液质量浓度0.04 g/m L,以2 BV/h的吸附速率进行吸附,上样量为11 BV,用3 BV蒸馏水除杂后,以50%乙醇溶液为洗脱剂,洗脱液用量为4 BV,洗脱流速控制在2 BV/h。经AB-8树脂吸附富集后,样品总多酚质量浓度达到了30.11 mg/g,是粗提物(9.55 mg/g)的3倍。对纯化前后的榅桲总多酚进行PTP1B的抑制作用研究,结果显示,榅桲总多酚对PTP1B有较强的抑制作用,即榅桲总多酚纯化前的IC50为78.14μg/m L,用AB-8树脂纯化后所得总多酚的IC50为16.12μg/m L。

In this study,11 types of macroporous resins were screened for the purification of Cydonia oblonga Mill.polyphenols.The optimal resin was selected through static absorption and desorption experiments.Furthermore,its dynamic absorption features and influencing factors were analyzed.The results showed that the optimal conditions for purification were as follows：macroporous resin AB-8 had the best separation efficiency when the concentration of sample solution was 0.04 g/mL,the adsorption flow rate was 2 BV/h,the loading weight was 11 BV,removing impurities by 3 BV of distilled water and eluting the total polyphenols with 4 BV of 50% ethanol-water at flow rate of 2 BV/h.The concentration of total polyphenols of the product after the adsorption concentrating on AB-8 resin achieved 30.11 mg/g,which was 3 times of crude extract （9.55 mg/g）.The PTP1B inhibition effect of polyphenol extracts before and after the purification process was also discussed.The results indicated that,the total polyphenols of C.oblonga had strong inhibition effect on PTP1B with IC50 value of 16.12 μg/mL after purification by AB-8 resin and the IC50 value was 78.14 μg/mL before purification.