养荣润肠舒合剂对慢传输型便秘模型大鼠结肠中c-Kit、SP与VIP的影响

The Effects of Yangrong Runchangshu Mixture on c-Kit,SP and VIP in the Colon of Rats with Slow Transit Constipation

目的:研究养荣润肠舒合剂对慢传输型便秘(STC)模型大鼠结肠Cajal间质细胞中酪氨酸激酶受体蛋白CD117(c-Kit)、P物质(SP)与肠肌间神经丛血管活性肠肽(VIP)的影响。方法:sc盐酸吗啡(2.5 mg/kg),每天1次,连续45 d以复制大鼠STC模型。120只大鼠随机均分为正常对照(等容生理盐水)组、模型(等容生理盐水)组、麻仁丸(阳性对照,14.7 g/kg)组与养荣润肠舒合剂高、低剂量[15、7.5 g(生药)/kg]组。复制模型成功后ig给药,每天1次,连续10 d。采用免疫组化法染色,光镜下观察大鼠结肠c-Kit、SP、VIP分布,并测定其表达。结果:与正常对照组比较,模型组大鼠结肠c-Kit、SP阳性细胞数量减少、表达减弱,VIP阳性数量增加、表达增强,差异有统计学意义(P<0.01),细胞形态出现明显异常;与模型组比较,麻仁丸组和养荣润肠舒合剂高、低剂量组大鼠结肠c-Kit、SP阳性细胞数量增加、表达增强,VIP阳性细胞数量减少、表达减弱,差异有统计学意义(P<0.05),细胞形态有一定改善。结论:养荣润肠舒合剂对STC模型大鼠结肠中SP、VIP含量及c-Kit的表达有一定改善作用。

OBJECTIVE：To study the effects of Yangrong runchangshu mixture on tyrosine kinase receptor protein CD117（c-Kit）,substance P（SP）and myenteric nerve plexus vasoactive intestinal peptide（VIP）in the colon interstitial cell of Cajal of rat with slow transit constipation（STC）. METHODS：Morphine hydrochloride（2.5 mg/kg）was given sc once daily for 45 consecutive days to establish STC rat models. 120 rats were randomized into a normal control（isometric normal saline）group,a model（isometric normal saline）group,Maren pill（positive control,14.7 g/kg）group and Yangrong runchangshu mixture high-dose and low-dose [15 and 7.5 g（medicinal materials）/kg] groups. After successful establishment of the models,drugs were given ig once daily for 10 consecutive days. Immunohistochemical method was employed for staining. The changes in the structure and c-Kit,SP and VIP expressions in rats＇ colonic tissues were observed under the light microscope. RESULTS：Compared to the normal control group,those in the model group demonstrated weaker expressions of c-Kit and SP and less positive cells in colon,stronger expression of VIP and more positive cells,with statistical significances（P〈0.01）;cellular morphology became abnormal obviously.Compared to the model group,those in Maren pill group and Yangrong runchangshu mixture high-dose and low-dose groups showed stronger expressions of c-Kit,SP and more positive cells in colon,weaker expressions of VIP and less positive cells with statistical significances（P〈0.05）;cellular morphology was improved to some extent. CONCLUSIONS：Yangrong runchangshu mixture can improve the contents of SP and VIP and the expression of c-Kit in rats with STC to some extent.