三七活血片抗软组织损伤、镇痛与抗炎药理作用研究

Study on the Anti-soft Tissue Injury,Antalgic and Anti-inflammatory Pharmacological Actions of Sanqi Huoxue Tablets

目的:研究三七活血片的抗软组织损伤、镇痛与抗炎药理作用。方法:通过大鼠软组织损伤模型观察三七活血片(2.0、1.0、0.5 g/kg)的抗软组织损伤作用,进行损伤证候评分并测定大鼠血清中前列腺素E2(PGE2)、白细胞介素6(IL-6)含量;通过小鼠热板、扭体实验(2.0、1.0、0.5 g/kg)观察三七活血片的镇痛作用,测定小鼠舔足次数、扭体次数及痛阈值;通过小鼠足肿胀、耳肿胀实验观察三七活血片的抗炎作用,测定小鼠足肿胀度、耳肿胀度。结果:2.0、1.0 g/kg剂量下,三七活血片可降低模型大鼠损伤证候评分,可减少小鼠足肿胀度和耳肿胀度;2.0、1.0、0.5 g/kg剂量下,三七活血片可减少模型大鼠血清中PGE2、IL-6含量与小鼠舔足次数、扭体次数,升高痛阈值。结论:三七活血片具有一定的镇痛、抗炎的作用,对软组织损伤有较好的修复作用。

OBJECTIVE：To study anti-soft tissue injury,antalgic and anti-inflammatory pharmacological actions of Sanqi huoxue tablets. METHODS：The anti-soft tissue injury of Sanqi huoxue tablets（2.0,1.0,0.5 g/kg）were observed with soft tissue injury model rat,the damage syndrome score was calculated,and the contents of prostaglandin E2（PGE2）and interleukin 6（IL-6）were determined. Mouse hot-plate test and mouse writhing test（2.0,1.0 and 0.5 g/kg）were conducted to observe the antalgic effects of Sanqi huoxue tablets. Licking foot times,twisting times and the pain threshold value were determine. Mouse paw swelling test and mouse auricle swelling test were carried out to observe the anti-inflammatory effect. Paw swelling and auricle swelling were determined. RESULTS：2.0 and 1.0 g/kg Sanqi huoxue tablets decreased the damage syndrome score,reduced paw swelling and auricle swelling. 2.0,1.0 and 0.5 g/kg Sanqi huoxue tablets reduced the contents of PGE2 and IL-6 in serum of rats and licking foot times and twisting times,and increased the pain threshold value of mice. CONCLUSIONS：Sanqi huoxue tablets have antalgic and anti-inflammatory effects and can repair the soft tissue injury.