藤梨根乙酸乙酯提取物对肺癌A549细胞凋亡的诱导作用

Induction of Ethyl Acetate Extract from Actinidia arguta on Apoptosis of Lung Cancer A549 Cells

目的:研究藤梨根乙酸乙酯提取物对肺癌A549细胞凋亡的诱导作用。方法:以0(阴性对照)、40、80、160μg/ml藤梨根乙酸乙酯提取物培养细胞48、72 h后,凝胶电泳法测定细胞DNA裂解情况。以0(阴性对照)、40、80、160μg/ml藤梨根乙酸乙酯提取物培养细胞24、48、72 h后,流式细胞仪测定细胞凋亡和细胞周期分布情况;免疫组化法测定细胞生存素(Survivin)表达。结果:40、80、160μg/ml藤梨根乙酸乙酯提取物培养细胞48、72 h后出现凋亡细胞特有的梯状条带;与阴性对照比较,40、80、160μg/ml藤梨根乙酸乙酯提取物培养细胞24、48、72 h后细胞凋亡率升高,G0/G1期细胞比例升高,Survivin表达减弱,且呈时间、浓度依赖关系。结论:藤梨根乙酸乙酯提取物可诱导A549细胞凋亡,将细胞周期阻滞于G0/G1期,其机制可能与降低Survivin表达有关。

OBJECTIVE：To study the induction of ethyl acetate extract from Actinidia arguta on apoptosis of lung cancer A549 cells. METHODS：After the cells were cultured in 0（negative control）40,80 and 160 μg/ml ethyl acetate extract from A. arguta for 48 and 72 h,gel electrophoresis method was used to detect DNA cleavage in the cells. After the cells were cultured in 0（negative control）,40,80 and 160 μg/ml ethyl acetate extract from A. arguta for 24,48 and 72 h,flow cytometry was adopted to detect apoptosis and cell cycle distribution,and immunohistochemical method was employed to detect Survivin expression. RESULTS：After 48 and 72 h culture in 40,80 and 160 μg/ml ethyl acetate extract from A. arguta,ladders appeared,which are the characteristic of apoptosis. Compared to the negative control,following 24,48 and 72 h culture of cells in 40,80 and 160 μg/ml ethyl acetate extract from A. arguta,apoptosis rate was higher,also was the percentage of the cells in G0/G1phase;and the expression of Survivin was weaker,demonstrating a time and concentration-dependent relation. CONCLUSIONS：The ethyl acetate extract from A. arguta can induce apoptosis of A549 cells and cause a cell cycle arrest in G0/G1 phase,by a mechanism which may be related to the reduction in Survivin expression.