连参律平滴丸抗心律失常的动物实验研究

Experimental Study of Anti-arrhythmic Effects of Lianshen Lyuping Dripping Pills in Animals

目的:研究连参律平滴丸抗大鼠、小鼠、豚鼠心律失常的作用。方法:48只SD大鼠随机均分为正常对照(等容生理盐水)组、模型(等容生理盐水)组、心律平(阳性对照,270 mg/kg)组与连参律平滴丸高、中、低剂量(210、140、70 mg/kg)组;一次性尾iv给药10 min后尾iv乌头碱(10 mg/L,3μg/min),记录心电图(ECG)出现室性早搏(VP)、室性心动过速(VT)、室颤(VF)时的乌头碱用量。48只豚鼠分组、给药同上,一次性尾iv给药10 min后尾iv哇巴因(1 mg/L,1μg/min),记录出现VP、VT、VF时的哇巴因用量。120只KM小鼠分组、给药剂量同上,一次性ip给药30 min后置小鼠于含4 ml氯仿的烧杯中,呼吸停止后开胸观察并计算室颤率。120只Wistar大鼠分组、给药剂量同上,舌下iv 0.8%Ba Cl2(0.25 ml/kg),待出现典型心律失常后一次性舌下iv给药;48只Wistar大鼠分组、给药剂量同上,舌下iv 5%Ca Cl2,出现典型心律失常后一次性舌下iv给药,测定大鼠窦性心律失常恢复率。48只SD大鼠分组、给药剂量同上,ig给药,每天1次,连续7 d后复制大鼠心肌缺血再灌注模型,记录VT、VF发生率及死亡率。结果:与正常对照组比较,模型组大鼠各项指标均异常。与模型组比较,连参律平滴丸高、中、低剂量组动物出现VP、VT、VF时乌头碱/哇巴因用量增加,小鼠室颤率降低,大鼠窦性心律失常恢复率升高;连参律平滴丸高、中剂量组大鼠VT、VF发生率和死亡率均降低,差异有统计学意义(P<0.01或P<0.05)。结论:连参律平滴丸对动物具有一定的抗心律失常作用。

OBJECTIVE：To study anti-arrhythmic effects of Lianshen lyuping dripping pills in rats,mice,guinea pigs. METHODS：48 SD rats were randomized into a normal control（isometric normal saline） group,a model（isometric normal saline）group,a propafenone（positive control,270 mg/kg）group and Lianshen lyuping dripping pills high-dose,medium-dose and lowdose（210,140,70 mg/kg）groups. The rats＇ tails were given the drugs iv at a time,10 min later,aconitine（10 mg/L,3 μg/min）iv. The doses of aconitine were recorded when ventricular premature beat（VP）,ventricular tachycardia（VT）and ventricular fibrillation（VF）developed in ECG. 48 guinea pigs were grouped and given drugs as above and 10 min later,were given uabain iv（1mg/L,1 μg/min）. The doses of uabain were recorded when VP,VT and VF developed in ECG. 120 KM mice were grouped and given drugs ip at a dose as above at a time,30 min later,were placed in the beaker containing 4 ml chloroform. When respiratory arrest occurred,ventricular fibrillation rate of mice were calculate.120 Wistar rats were grouped and given drugs at a dose as above,following typical arrhythmia reaction,given 0.8% Ba Cl2（0.25 ml/kg）iv and sublingually at a time;48 Wistar rats were grouped and given drugs at a dose as above and,following typical arrhythmia reaction, given 5%Ca Cl2（0.28 ml/kg）iv and sublingually at a time. Recovery of sinus arrhythmia rate of rats were determined.48 SD rats were grouped and given drugs at a dose as above,after the administration ig once daily for 7 consecutive days,the rat models of myocardial ischemia reperfusion were established,and then VT,VF incidence rate and death were recorded. RESULTS：Compared to the normal control group,those in the model group were all abnormal in all indexes. Compared to the model group,those in Lianshen lyuping dripping pills high-dose,medium-dose and low-dose groups used more aconitine and uabain at the time of the occurrence of VP,VT and VF,had a lower occurrence rate of VF in mice and higher recovery of sinus arrhythmia rate in rats. The rats in Lianshen lyuping dripping pills high-dose and medium-dose groups which had myocardial ischemia reperfusion demonstrated a lower of VT,VF incidence rate and death rate. There were statistically significances（P〈0.01 or P〈0.05）.CONCLUSIONS：Lianshen lyuping dripping pills have an anti-arrhythmic effects in animals to some degree.