五味子果实和藤茎提取液脱色剂的筛选

Screening of Decolorizing Agents for the Extracts from the Fruit and Stem of Schisandra chinensis

目的:筛选适合五味子果实和藤茎提取液的脱色剂。方法:采用高效液相色谱法测定使用8种脱色剂(活性白土、活性炭、硅藻土、钙质膨润土、高岭土、活性氧化铝、氧化镁、凹凸棒土)对五味子果实和藤茎提取液脱色后4种木脂素成分(五味子醇甲、五味子醇乙、五味子甲素、五味子乙素)的含量,分别计算五味子果实和藤茎样品的脱色率和各木脂素成分的保留率。结果 :8种脱色剂对五味子果实和藤茎提取液的脱色效果大小依次为凹凸棒土>活性炭>活性氧化铝>高岭土>氧化镁>硅藻土>钙质膨润土>活性白土,活性炭>硅藻土>凹凸棒土>氧化镁>高岭土>活性氧化铝>活性白土>钙质膨润土;其中凹凸棒土和活性炭分别对五味子果实和藤茎提取液脱色效果最好,脱色率分别为60.47%、69.24%;对五味子醇甲、五味子醇乙、五味子甲素、五味子乙素的保留率分别为77.43%、77.73%、77.07%、77.53%和72.18%、70.17%、70.32%、70.28%。结论:在试验的8种脱色剂中,分别以凹凸棒土和活性炭对五味子果实和藤茎提取液的脱色效果最优。

OBJECTIVE： To screen decolorizing agents suitable for the extracts from the fruit and stem of Schisandra chinensis. METHODS： HPLC was adopted to determine the contents of 4 kinds of lignans （schizandrin, schisandrol B, deoxyschizandrin and γ-schizandrin） in the extract solution from the fruit and stem of S. chinensis which was treated with 8 kinds of decolorizing agents （activated clay, activated carbon, diatomite, calcium bentonite, kaolin, activated aluminium oxide, magnesium oxide, attapulgite clay）, and the decolourization rates of the samples of the fruit and stem of S. chinensis and the retention rates of lignans in such samples were calculated respectively. RESULTS： The above-mentioned decolorizing agents were arranged in order as follows respectively based on the decolourization effects on the extract solution from the fruit and stem of S. chinensis： attapulgite clay〉activated carbon〉activated aluminium oxide〉kaolin〉magnesium oxide〉diatomite〉calcium bentonite〉activated clay, and activated carbon〉diatomite〉attapulgite clay〉magnesium oxide〉kaolin〉activated aluminium oxide〉activated clay〉calcium bentonite. The attapulgite clay and activated carbon have the best decolourization effects on the extracts from the fruit and stem of S. chinensis, with the decolourization rates of 60.47% and 69.24% respectively, and the retention rates of schizandrin, schisandrol B, deoxyschizandrin and γ-schizandrin were 77.43%, 77.73%, 77.07%, 77.53%, and 72.18%, 70.17%, 70.32%, 70.28%, respectively. CONCLUSIONS： Among the 8 decolorizing agents, attapulgite clay and activated carbon have the best decolourization effects on the extract solution from the fruit and stem of S. chinensis.