猪病轻简化实用快速诊断技术与应用

Development and Application of Immunochromatographic Lateral Flow Strip Tests for Rapid Diagnosis of Swine Diseases

诊断是有效控制和消灭疫情的基础,准确的诊断结果是制定动物疫病防控措施的科学依据,动物疫病诊断技术是反映动物防疫整体水平的重要指标。免疫层析试纸快速检测技术具有操作简便、快捷、无需特殊仪器设备、检测结果清楚直观、试剂稳定和便于保存等特点,适用于动物疫病现场快速检测,对猪病诊断、指导免疫和建立适宜的免疫程序等有重要作用。国家生猪产业技术体系自启动以来,以实现猪病检测技术的快速、灵敏、特异、简便化为目标,疫病控制研究室围绕重要猪病病原,重点开展了免疫层析试纸技术研究,成功研制了一批具有自主知识产权的抗体快速检测技术产品,获得国家二类新兽药证书2项,河南省科技进步一等奖1项,每年向国家生猪产业技术体系综合试验站,基层兽医站以及不同类养猪企业免费提供猪病快速检测试纸4万条以上,为防控生猪疫情提供了技术支撑。

Diagnosis is basic for the effective control and elimination of swine diseases. Science and technology level for diagnosis is an essential indicator for prevention and control swine diseases. Immunochromatographic Lateral-flow Strip test is the best representative for point-of-care diagnosis, possessing the properties of easy-to-use, quickness, high sensitivity and specificity, and eliminating the use of expensive equipment. It is stable, convenient, and can provide visible results within 5 min, especially suitable for detecting animal diseases at the point of care. National Swine Industry Technology System holds the goal of detecting swine diseases with more simple, sensitive, specific and rapid methods since its launching. In the light of this goal, the Laboratory for Disease Control developed immunochromatographic lateral-flow strip tests for the detection of many swine diseases. A number of antibody detection strip with independent intellectual property rights have been developed. Due to the simplicity, high specificity and sensitivity, strip tests technology have won 2 national class B new veterinary medicine registration certificates and 1 provincial class A reward for scientific local veterinary stations and swine herds of various scales and technological progress. We provided 40 000 strips for every year, providing an important support for preventing and control of swine diseases.