人抗增殖蛋白1生物信息学分析

Bioinformatics analysis of human prohibitin

目的对人抗增殖蛋白1(PHB1)进行生物信息学分析,预测其结构和功能,为人PHB1的功能研究和利用奠定基础。方法利用生物信息学工具对人PHB1的染色体定位、跨膜区域、空间结构、理化性质和功能区进行预测。结果人PHB1由272个氨基酸组成,其中丙氨酸含量最高。该蛋白等电点为5.57,相对分子质量为29 804.1,分子式为C1331H2154N370O400S2。该蛋白为非跨膜的疏水蛋白,主要由α-螺旋构成。结论人PHB1为细胞膜蛋白超家族成员,发挥相应的生物学功能,也参与了人类许多疾病的发生、发展过程。

Objective To perform the bioinformatics analysis for predicting the structure and function of human pro- hibitin 1 （PHB～） to lay the foundation for its functional research and application. Methods The bioinformatics tools were used to predict the chromosome location, transmembrane region, spatial structure, physical and chemical properties and functional regions of PHBl. Results The bioinformatic analysis revealed that PHB1 was composed of 272 amino acids,in which the alanine content was highest;the theoretical isoelectric point was 5.57, the molecular formula was C1331H2154N370O400S2 with a relative molecular mass of 29 804.1. PHB1 protein was a non-transmembrane hydrophobin,which was constituted by alpha-helix. Conclusion Human PHBt is a superfamily member of cellular membrane protein,plays the corresponding biological function and also participate in the occurrence and development of many human diseases.