水稻miR169o及其靶基因OsNF-YAs对缺水胁迫的早期表达模式

Dynamic Expression of miR169o and Its Target Genes OsNF-YAs in the Early Response to Water Deficiency in Rice

Micro RNAs(mi RNAs)是一类小的非编码RNA,在植物逆境胁迫应答中发挥重要的调控作用。mi R169可受干旱胁迫诱导表达,而过表达mi R169则可以增强植物对干旱的耐受性。然而,mi R169及其靶基因NF-YAs在水稻干旱胁迫条件下的表达动态至今尚不清楚。对水稻进行不同时间缺水处理,用q RT-PCR法定量测定了水稻根、茎、叶组织中mi R169o及其靶基因表达的动态变化。结果表明,随着缺水处理时间的增加,水稻不同组织中mi R169o表达量总体上有升高趋势;而靶基因(NF-YA1、NFYA2和NF-YA3)表达模式基本与mi R169o的表达模式相反,但并不全部对应。推测在水稻干旱胁迫早期反应中,mi R169o可能主要调控了部分特定靶基因的表达。此外,mi R169o在水稻根、茎、叶组织中的表达和丰度存在着明显的差异,具有组织特异性。

MicroRNAs （ miRNAs ） is noncoding RNAs and play important roles in plant development and response to various environment stresses. Recent evidence have indicated that miR169 is upregulated response to drought stress, and overexpression of miR169 contributes to improve the tolerance to drought stress in plant. However, it is still unclear to the expression pattern of miR169 under drought stress and the regulatory mechanism response to drought stress in rice. In this study, the dynamic expression patterns of miR169o and its target gene NF-YAs （ Nuclear Factor Y A subunit, NF-YA ） in the root, stem and leaf of rice were analysed systematically by qRT-PCR after water deficiency treatment. Generally, miR169o showed up-regulated after water deficiency stress treatment compared to that before treatment, while target gene NF-YAs showed imperfect reverse expression pattern to miR169o. In addition, the expression pattern and abundance of miR169o in rice root, stem and leaf suggested that the expression of miR169o is tissue-specific.