海洋真菌提取物损伤大肠杆菌DNA活性的筛选及活性菌株研究

Screening the Extracts of Marine Fungi for the Activity of Damaging the DNA of Escherichia coli and Researches on Active Strains

从海洋微生物中筛选抗菌、抗肿瘤物质是海洋药物研究的热点之一,而筛选模型在研究中起着重要作用。建立了可用于抗菌及潜在抗肿瘤活性物质筛选的大肠杆菌差异性DNA修复试验模型,并运用该模型对海洋共附生真菌的提取物进行了筛选。结果显示,5株海洋真菌的提取物具有较强的选择性抑制DNA损伤修复基因缺陷型大肠杆菌的活性,可能具有DNA损伤作用。对这5种活性真菌进行了分子生物学鉴定,而且薄层色谱(TLC)和高效液相色谱(HPLC)-活性追踪显示这些菌株可产生较多样化的活性物质。

Screening of antimicrobial and antitumor agents from marine microorganisms is one of the hot spots for the research of marine drugs, and screening models play an important role in the study. The authors set up an Escherichia coli differentiated DNA damage repair test model, which can be used for the screening of antimicrobial and potential antitumor substances. This model was further applied to screen the bioactivities of the extracts of marine symbiotic fungi. As the result, five marine fungal strains＇ extracts showed strong selective inhibition to E. coli strains with DNA damage repairing genes-deficiency, i.e, the potential of damaging DNA. The five strains were identified by molecular biological methods. Furthermore, analyses by thin layer chromatography （ TLC ）and high performance liquid chromatography（ HPLC ）-bioactivity tracing revealed that these strains produced diverse bioactive substances.