东北地区产油能源微藻的筛选和鉴定

Selection and Identification of Oil-producing Energy Microalgae in Northeast Region

微藻作为21世纪生物柴油的理想燃料已被人们广泛的关注,但是目前微藻种类很多,如何从诸多的微藻中筛选油脂含量高的微藻已成为人们函待解决的问题。从东北地区水样中分离纯化出93种藻种,采用尼罗红染色法对其中30株藻种进行了筛选获得了8种具有产油潜力的藻种,并利用自制的柱式反应器微藻评价装置对这8株藻株进行了产油能力的评价,获得了一株总脂产率达到133.9 mg/(L·d)的产油能源微藻。在此基础上,对该藻株进行了18S r RNA的鉴定,确定为Chlorella sp.。

In the 21st century, the ideal microalgae biodiesel fuel has been widespread concern, but how to filter high in fat content from a lot of microalgae species has become a problem to be addressed. In this study, 93 strains of microalgae were isolated and purified from the water samples of the Northeast region, and 30 strains were screened to obtain eight oil-producing algae by Nile red fluorescence. Oil production capacity of these eight microalgae were evaluated with bubble column photobioreactor. This experiment obtained a higher oil yield algal and its total oil yield reached 133. 9 mg / （ L · d ） . On this basis an oil-producing energy microalgae was identificated by 18S rRNA. This oil-producing energy microalgae was identificated as Chlorella sp.