夹脊电针对脊髓损伤后大鼠c-fosmRNA及BNIP3 mRNA表达影响的实验研究

Effect of Jiaji Electro-acupuncture on Expressions of c-fos Gene and NIP3 mRNA in the Injured Spinal Cord of Rats

目的:观察夹脊电针对大鼠脊髓继发性损伤后c-fos mRNA及BNIP3 mRNA表达及其变化规律,以及对CBS运动功能评分的影响,观察夹脊电针对脊髓损伤后神经细胞的保护作用及凋亡机制。方法:用改良Alle’s重物坠落法制备大鼠脊髓损伤后模型,在各时间点观察各组不同时间脊髓内c-fos mRNA及BNIP3 mRNA表达水平以及CBS评分的变化。结果:CBS评分结果显示:7天后,夹脊电针组大鼠的动作完成明显优于假手术组和模型组,具有显著性差异。c-fos mRNA阳性细胞数量表达结果显示:假手术组脊髓内存在少量c-fos mRNA弱阳性的神经细胞,且3个时间点表达的变化不显著;模型组和夹脊电针组的c-fos mRNA表达,在术后均迅速升高,在术后第1天时明显升高,第3天时,表达逐渐下降,第7天时有所回升;夹脊电针组和模型组在各时间点的c-fos mRNA表达阳性细胞数量,与假手术组相比差异具有显著意义(P<0.05);术后第1天时,夹脊电针组与模型组相比,c-fos mRNA表达阳性细胞数低于模型组;术后第3天时,夹脊电针组与模型组相比,不具有显著差异;术后第7天时,夹脊电针组与模型组相比,c-fos mRNA表达阳性细胞数高于模型组。BNIP3 mRNA表达结果显示:夹脊电针组内比较,治疗7天组与治疗3天组相比,BNIP3 mRNA表达水平明显降低(P<0.05),差异具有统计学意义;夹脊电针组与模型对照组相比,治疗7天时,BNIP3 mRNA表达水平有明显降低(P<0.05),具有统计学意义。结论:夹脊电针可促进脊髓损伤后肢体运动、感觉功能的恢复,并通过影响c-fos mRNA及BNIP3 mRNA的表达,促进脊髓损伤后功能的恢复及抑制神经细胞的凋亡。

Objective：To probe into the effect and the change rule of Jiaji electro - acupuncture in the treat- ment of injured spinal cord by observing the number of positive cells of c - fos gene and NIP3 mRNA , and the impact on the CBS movement function score. To further explore the protective mechanism of Jiaji electro - acu- puncture on nerve cells for injured spinal cord and the action of c - fos gene and NIP3 mRNA. Methods：45 healthy male Wistar rats were divided into 3 groups ：a Jiaji electro - acupuncture group（ n = 15 ）, a model control group （n = 15 ）, a sham operation group（ n = 15）. Lamina of vertebra was cut without spinal cord injury in the sham - operation group. Injury of T10 spinal cord was induced by modified Alien＇s method in other two groups. Observe the expressions of c - fos mRNA and NIP3 mRNA. The expressions of c - fos mRNA and NIP3 mRNA,the changes of CBS movement function score in each group were observed for 1,3 and 7days after spinal injury. Results： The CBS movement function score showed that after 7 days, the complete action of Jiaji electro - acu- puncture group was better than the model control group and sham operation group. The result of the number of positive cells of c - los gene showed that there was a small amount of c - los mRNA weakly positive neurons in sham operation group and had no significant difference at 3 time points. The expression of mRNA of c - los gene increased in postoperative in Jiaji electro -acupuncture group cantly on the first day after operation. The expression graduall seventh day;on the first day after operation, the expression and the model control group. It increased signifi- y declined on the third day and picked up on the of mRNA of c - fos gene of Jiaji electro - acupunc- ture group was lower than that in the model group （ P 〈 0.05 ） ; on the third day, there was no significant differ- ence between Jiaji electro -acupuncture group and model group;on the seventh day, the expression of mRNA of c - fos gene of Jiaji electro - acupuncture group was higher than that in the model group （ P 〈 0. 05 ）. The result of NIP3 gene showed that as to the acupuncture in the treatment group, 7 d treatment group and 3 d group were compared and the BNIP3 mRNA expression levels decreased obviously （ P 〈 0. 05 ） ; on the seventy day after operation, the expression of mRNA of BNIP3 gene of Jiaji electro - acupuncture group was lower than that in the model group （ P 〈 O. 05 ）. Conclusion： The motor function and sensory function of rats with acute spinal cord in- juries can be improved by Jiaji electro -acupuncture. C -fos mRNA and BNIP3 mRNA were impacted by Jiaji electro - acupuncture, suggesting that c - fos mRNA can promote the recovery of function and inhibition of nerve cell apoptosis of the rats with acute spinal cord injuries.