艾灸对胰腺癌裸鼠移植瘤DPC4、ki67表达的影响

Effect of Moxibustion on DPC4 and ki67 Expression of Pancreatic Carcinoma in Nude Mice

目的:观察艾灸对胰腺癌荷瘤裸鼠移植瘤DPC4、ki67表达影响的实验研究,并探讨艾灸抗胰腺癌的可能作用机制。方法:将16只裸鼠均接种Bx PC-3细胞构建胰腺癌裸鼠移植瘤的动物模型,随机分为模型组和艾灸组。接种后次日给予麦粒灸裸鼠大椎穴至14天后结束治疗,取出肿瘤组织。采用免疫组化法检测肿瘤组织中DPC4、ki67蛋白的表达。结果:与模型组相比,艾灸组中DPC4蛋白表达升高,而ki67表达显著降低(P<0.05)。结论:艾灸对肝癌裸鼠移植瘤的生长有明显的抑制作用,其机制可能与上调肿瘤组织中DPC4、下调ki67表达水平有关。

Objective：To observe the efficacy of DPC4 and ki67 in the expression of pancreatic carcinoma in nude mice treated by moxibustion and explore the possible mechanism of antitumor through moxibustion.Meth-ods：16 nude mice were inoculated with BxPC-3 cells to construct pancreatic carcinoma xenograft models and were divided into a model group and a moxibustion group randomly.On the next day give mice moxibustion at Dazhui point.After the treatment for 14 days,the tumor tissues in nude mice were removed.Use immunohisto-chemical method to detect the protein expression of DPC4 and ki67 in tumor tissues.Results：Compared with the model group,the DPC4 expression was significantly higher and ki67 expression was lower （P〈0.05）.Con-clusion：Moxibustion has a significant inhibition on the growth of transplanted tumor and the mechanism may be related to the up-regulation of the expression level of DPC4 and down-regulation of the expression level of ki67 in tumor tissues.