摘要
目的:旨在探讨深圳市乙肝患者 HBV-DNA 与免疫球蛋白(IgA,IgG,IgM)和补体(C3,C4)的关系。方法首先采用实时荧光定量 PCR 技术检测210例乙肝患者的 HBV-DNA,按检测结果10n copies/ml 进行分组,可分为102~108 copies/ml 7组实验组。然后对7组实验组和对照组同时进行 IgA,IgG,IgM 及 C3,C4检测,分析乙肝患者 HBV-DNA 与IgA,IgG,IgM 及 C3,C4的相关性。结果乙肝患者 HBV-DNA 为105~108 copies/ml 时,均出现了 IgA,IgG,IgM 的增高(U =12.43,10.96,6.42,P <0.01)及 C3,C4的降低(U =8.37,6.0,P <0.01),而在102~104 copies/ml 只出现 IgA 和 IgM的增高(U =2.36,2.04,P <0.05),其他指标差异均无统计学意义。在7组实验组的两两比较中,只有104组与105组出现了显著性的变化(IgA,IgM 增高,C4降低;U =2.39,2.46,2.09,P <0.05,IgG 增高,U =3.25,P <0.01),而其他低载毒量组之间和高载毒量组之间均无显著性差异。结论乙肝患者体内不同的 HBV 载量会引起 IgA,IgG,IgM 及 C3,C4相应的改变,特别是当 HBV 载量为105~108 copies/ml 的4个实验组,伴随着 HBV-DNA 的增高均出现 IgA,IgG,IgM 增高及C3,C4降低,严重的影响了机体免疫功能,两者呈正相关(r =0.967,P <0.01)。104组与105组各指标出现显著性改变,提示对乙肝患者的抗病毒护肝治疗中,要尽量将 HBV 载量控制在104 copies/ml 以下,才能达到最小程度损伤免疫功能的疗效。
Objective To investigate the relationship between Hepatitis B patients with different viral loads and immunoglob-ulin A,G,M and complement C3,C4.Methods Firstly,followed by real-time fluorescence quantitative PCR detection 210 cases of hepatitis B patients with HBV-DNA levels,according to 10n copies/ml different viral load detection results,it was divided into 102 ~108 copies/ml of the experimental groups.Then the experimental groups and control group were simulta-neously detected in immunoglobulin A,G,M and complement C3,C4.Analysed the correlation between HBV loads and im-munoglobulin A,G,M and complement C3,C4.Results When the viral loads of hepatitis B patients were 105 ~108 copies/ml,the testing results of IgA,IgG and IgM were both increasing (U =12.43,10.96,6.42,P 〈0.01),while C3 and C4 were both decreasing (U =8.37,6.0,P 〈0.01).When the viral loads of hepatitis B patients were 102 ~ 104 copies/ml,only IgA and IgM were increasing (U =2.36,2.04,P 〈0.05),the other testing results had no statistical significance.Between the test of 7 experimental groups compared with each other,only 104 group and 105 group had significantly changed (IgA and IgM were increasing,C4 was decreasing,U =2.39,2.46,2.09,P 〈0.05,IgG was increasing,U = 3.25,P 〈0.01),but between other low viral loads or high viral loads were not significantly differences.Conclusion The different viral loads of hepatitis B patients could cause the different changes of immunoglobulin A,G,M and complement C3,C4,especially in the 4 groups from 105 to 108 copies/ml.Followed by increasing in viral loads,there were immunoglobulin A,G,M increasing and comple-ment C3,C4 decreasing,and also serious impaction on the immune function of organism.There was a positive correlation be-tween viral loads in vivo and immune damages,correlation coefficient (γ =0.967,P 〈0.01).When the viral loads from 104 to 105 copies/ml,the testing results had changed significantly.It suggest that should control viral loads under 104 copies/ml in the hepatitis B antiviral treatment,so the effect of immune function damage will be the minimum.
出处
《现代检验医学杂志》
CAS
2015年第4期67-70,共4页
Journal of Modern Laboratory Medicine
基金
2014年深圳市福田区卫生公益性科研项目(FTWS2014006).