MMP-2、MMP-7和MMP-14在胃癌患者组织中表达的临床意义

Expression and clinical significance of MMP-2,MMP-7 and MMP-14 in the tissue of patients with gastric cancer

目的探讨胃癌组织中MMP-2、MMP-7和MMP-14的表达及临床意义。方法应用链霉素抗生物素蛋白-过氧化酶免疫组化染色法(S-P)法检测98例胃癌组织中MMP-2、MMP-7和MMP-14蛋白的表达。对照组20例为良性胃病变黏膜组织。结果胃癌组中MMP-2、MMP-7和MMP-14表达的阳性率明显高于对照组(P<0.05);胃癌组中MMP-2及MMP-14的阳性表达率与胃癌的肿瘤浸润深度、淋巴转移、肿瘤大小、大体分型、临床肿瘤分期有关(P<0.05或P<0.01)。MMP-7的阳性表达率与胃癌的肿瘤浸润深度、淋巴转移、分化程度、大体分型、临床肿瘤分期有关(P<0.05或P<0.01)。MMP-2和MMP-7蛋白表达正相关性(r=0.271,P<0.01);MMP-2和MMP-14蛋白表达正相关性(r=0.268,P=<0.01);MMP-7和MMP-14蛋白表达正相关性(r=0.202,P<0.05)。结论 MMP-2、MMP-7、MMP-14的表达上调与胃癌的浸润和转移有关。

Objective To investigate the expressions and clinical significance of MMP-2, MMP-7 and MMP-14 in the tissue of patients with gastric cancer. Methods The expressions of MMP- 2, MMP-7 and MMP-14 were detected by S-P immunohistochemical staining in gastric cancer （ n = 98 ） and normal control（n = 20）. Results In gastric cancer group, the positive expression rate of MMP- 2, MMP-7 and MMP-14 were significantly higher than those in normal control group（P 〈0.05）. The positive expression rate of MMP-2 and MMP-14 in gastric cancer were correlated with the depth of invasion, lymph node metastasis, tumor size, macroscopic type, tumor clinical stage （ P 〈 0.0 5 , P 〈 0.01 ）. The positive expression rate of MMP-7 in the gastric cancer was correlated with the depth of in- vasion, lymph node metastasis, differentiation, macroscopic type, tumor clinical stage （P 〈0.05, P 〈0.01 ）. The correlation analysis showed that there was positive correlation between expressions of MMP-2 and MMP-7 （ r = 0. 271, P 〈 0.01 ）. There was positive correlation between expression of MMP-2 and MMP-14 （ r = 0. 268, P 〈 0.01 ）. There was positive correlation between expression of MMP-7 and MMP-14 （r = 0. 202, P 〈 0.05 ）. Conclusion Up-regulation of MMP-2, MMP-7 and MMP-14 were correlated with invasion and metastasis of gastric cancer.