接合物蛋白在微小甲状腺乳头状癌组织中的表达及意义

Expression and significance of CRKL in thyroid papillary micro-carcinoma

目的：探讨接合物蛋白（CRKL）在微小甲状腺乳头状癌组织中的表达及其临床意义。方法收集120例患者的甲状腺组织石蜡标本，其中30例为直径＞1 cm 的甲状腺乳头状癌，30例为微小甲状腺乳头状癌，30例为结节性甲状腺肿，30例为未患甲状腺相关疾病患者的标本，采用免疫组化（SP）法检测标本中CRKL 的表达情况。结果微小甲状腺乳头状癌组、甲状腺乳头状癌组 CRKL 表达阳性率分别为30．12％、29．87％，高于结节性甲状腺肿组、正常甲状腺组的30．03％、28．57％（χ2＝52．102，P ＜0．05）；微小甲状腺乳头状癌组、甲状腺乳头状癌组平均吸光度（A）值分别为（0．516±0．100）、（0．496±0．201），均高于结节性甲状腺肿组、正常甲状腺组的（0．246±0．050）、（0．117±0．015），差异有统计学意义（F ＝149．105，P ＜0．05）。结论CRKL 在微小甲状腺乳头状癌中呈高度表达，临床上 CRKL 的检测有助于确定微小甲状腺乳头状癌的手术方案。

Objective To investigate the expression of CRKL in thyroid papillary micro-carcinoma and its clinical significance.Methods 120 patients with thyroid tissue specimens were collected,in which 30 cases of diam-eter 〉1 cm of papillary thyroid carcinoma,30 cases of thyroid papillary micro-carcinoma,30 cases of nodular goiter, 30 cases for specimen of thyroid disease patients without diabetes.Immunohistochemical（SP）method was used to de-tect samples in CRKL expression.Results In thyroid papillary micro-carcinoma and thyroid papillary cancer group, CRKL expression positive rates were 30.12% and 29.87% respectively,which were higher than that of nodular goiter and normal thyroid group 30.03% and 28.57%（χ2 =52.102,P 〈0.05）;The average absorbance（A）value in thy-roid papillary micro-carcinoma and thyroid papillary carcinoma group which were respectively （0.516 ±0.100）and （0.496 ±0.201）,were higher than that in nodular goiter and normal thyroid group （0.246 ±0.050）and （0.117 ±0.015）,the difference was statistically significant（F =149.105,P 〈0.05）.Conclusion CRKL is highly expressed in papillary thyroid micro-carcinoma and the clinical detection of CRKL is helpful to determine the surgical plan for papillary thyroid micro-carcinoma.