激光辅助孵化对玻璃化冻融囊胚妊娠结局的影响

The impact of laser assisted hatching on clinical outcomes of vitrification frozen-thawed blastocyst

目的探讨激光辅助孵化对玻璃化冻融的囊胚妊娠结局的影响。方法选取常规进行解冻移植囊胚的患者共495例,采用激光薄化法进行辅助孵化,根据不同年龄及是否孵化进行分组,比较各组间临床妊娠率、种植率及流产率。结果女性年龄≤36岁组中,AH组(孵化)与No AH组(未孵化)临床妊娠率及种植率差异不显著(AH vs No AH:57.7%vs52.1%;43.5%vs40.5%,P>0.05);女性年龄>36岁组中,AH组临床妊娠率及种植率高于No AH组,但无统计学差异(AH vs No AH:44.8%vs32.1%;32.1%vs24.5%,P>0.05)。两组中AH与ON AH流产率差异不显著。结论激光薄化法是一种安全可行的辅助孵化法,但不宜对所有的患者进行激光辅助孵化,尤其是年轻患者,可选择性对高龄妇女进行激光辅助孵化。

Objective：To investigate the influence of laser assisted hatching on pregnancy outcome in frozen-thawed blastocyst. Methods：Patients transplanting thawed blastocyst were divided into different groups according to different age and assisted hatching,comparing with the clinical pregnancy rate,implantation rate and abortion rate. Results：For the patients less than 36 years old,the clinical pregnancy rate and implantation rate of AH group and non-AH group is no difference（AH vs No AH：57.7%vs52.1%;43.5%vs40.5%,P〉0.05）;For the patients more than 36 years old,the clinical pregnancy rate and implantation rate of AH group is higher than non-AH group remarkable,but no significative difference（AH vs No AH：44.8%vs32.1%;32.1%vs24.5%,P〉0.05）.The abortion rate was no significant difference between two groups.Conclusion：The zona pellucida thinning is a safe and feasible method to assisted hatching,could use in elder patients selectively,but not suitable for all patients especially young women.