摘要
A series of novel coumarin glycoside esters（1-9） was synthesized through the acylation reaction of 4-methylcoumarin-7-O-β-D-glucoside（11） with different long chain fatty acids catalyzed by lipase in organic medium. The acylation occurred regioselectively at the 6＇-OH of glycosyl moiety. The enzymatic synthesis was optimized to achieve 54%-70% yield using immobilized lipase（Novozym 435, 10 mg/mL） as catalyst and acetone and pyri- dine（9：1, volume ratio, water content〈1%） as solvent with an acyl donor/coumarin glycoside molar ratio of 10：1 at a temperature of 40--50 ℃ for 96 h. All the synthesized compounds were confirmed.
A series of novel coumarin glycoside esters（1-9） was synthesized through the acylation reaction of 4-methylcoumarin-7-O-β-D-glucoside（11） with different long chain fatty acids catalyzed by lipase in organic medium. The acylation occurred regioselectively at the 6＇-OH of glycosyl moiety. The enzymatic synthesis was optimized to achieve 54%-70% yield using immobilized lipase（Novozym 435, 10 mg/mL） as catalyst and acetone and pyri- dine（9：1, volume ratio, water content〈1%） as solvent with an acyl donor/coumarin glycoside molar ratio of 10：1 at a temperature of 40--50 ℃ for 96 h. All the synthesized compounds were confirmed.
基金
Supported by the National Natural Science Foundation of China（Nos.21342015, J1210040） and the Hunan Provincial Natu- ral Science Foundation, China（No. 14JJ2048）.
