肝癌患者肿瘤坏死因子-α和白细胞介素-8水平变化及其与临床病理特征的关系

Changes of serum tumor necrosis factor-α and interleukin-8 in hepatocarcinoma patients and their relationship between them and clinicopathologic characteristics

目的探讨肿瘤坏死因子-α(TNF-α)和白细胞介素-8(IL-8)在肝癌中联合检测的临床意义,揭示二者变化与肝癌临床病理特征的关系。方法采集诊断明确的肝癌患者(肝癌组)术前、术后血清与健康人群(对照组)血清,并采集肝癌患者肝组织石蜡标本,采用酶联免疫吸附法检测血清TNF-α和IL-8水平,免疫组织化学法检测肝癌患者肝组织中TNF-α和IL-8的表达,分析其与临床病理特征的关系。结果肝癌患者术后血清TNF-α和IL-8水平较术前显著降低(P<0.01),但仍显著高于对照组人群(P<0.01)。肝癌患者血清TNF-α和IL-8水平与性别、年龄、组织类型、临床分期及肿瘤大小无明显相关性(P>0.05),与有无淋巴结转移有关(P<0.05)。肝癌组织中TNF-α和IL-8的表达呈正相关(P<0.01)。结论 TNF-α和IL-8可作为肝癌转移的重要标志物及预后评估的指标。

Objective To explore the clinical significance of combined detection of serum levels of tumor necrosis fac- tor-α（TNF-α） and interleukin-8（ IL-8 ） in hepatoearcinoma and the relationship between TNF-α, IL-8 and clinicopathologic characteristics. Methods Serum of hepatocarcinoma patients who had definite diagnosis （hepatocarcinoma group） before and after operation and serum of healthy people who had examination（ control group） were selected, and the hepatocareinoma tissue paraffin specimen was also selected. The serum levels of TNF-α and IL-8 were detected by enzyme linked immunosorbent assay and immunohistochemistry was used to detected the TNF-α and IL-8 expression in hepatocareinoma tissue. The relationship be- tween TNF-α, IL-8 and clinicopathologic characteristics was analyzed. Results Serum levels of TNF-α and IL-8 in hepatocar- cinoma group decreased more significantly after operation than those before operation（ P 〈 0. 01 ） , but still higher significantly than those in control group （P 〈 0. 01 ）. TNF-α and IL-8 levels had no obvious association with histological types, clinical stage and tumor size（ P 〉 0. 05 ）, but had relation with lymph node metastasis （P 〈 0. 05 ）. Serum level of TNF-α was positive corre- lated with IL-8 in hepatocarcinoma （ P 〈 0. 01 ）. Conclusion TNF-α and IL-8 may be used as an important metastasis markers for hepatocarcinoma and prognostic factors.