聚合酶链反应在流行性脑脊髓膜炎检测中的应用

Application of Polymerase Chain Reaction in Epidemic Cerebrospinal Meningitis detection method

目的应用聚合酶链反应(Polymerase Chain Reaction,PCR)技术对脑膜炎奈瑟菌(Neisseria meningitides)进行检测。方法应用脑膜炎奈瑟菌的的特异性DNA引物片断,对传统经典方法确诊的流脑病例阳性标本进行检测。结果 13例阳性流脑标本采用PCR方法检测与传统经典方法检测的结果一致;并且操作简便、快速、准确率高。结论为流脑的早期、快速诊断提供了一种技术手段。

Objective The application of polymerase chain reaction（PCR） for detection of Neisseria meningitides（Nm）. Methods Application of Neisseria meningitides specific DNA primer fragment, positive samples with traditional methods of epidemic cerebrospinal meningitis cases were detected. Results Results of 13 cases of epidemic cerebrospinal meningitis were positive by PCR detection method and the traditional method; and the operation is simple and rapid, with high accuracy. Conclusion For the early rapid diagnosis of epidemic cerebrospinal meningitis, and the technical support provided.