含硅再矿化液中硅含量对脱矿牙釉质再矿化影响研究

Effect of different concentration of silicon on demineralized enamel remineralization:a scanning electron microscopy study

目的观察不同质量分数含硅再矿化液对脱矿牙釉质表面微观形貌的影响,探讨其对脱矿牙釉质的再矿化作用。方法本研究于2014年4—8月在吉林大学口腔医院实验室进行。将牙釉质标本置于乳酸人工脱矿液中浸泡1周形成人工釉质龋,扫描电镜(SEM)观察确认人工釉质龋形成后,将脱矿釉质块随机分为5组,分别置于基础再矿化液(对照组)及含硅质量分数为0.5×10-6、1×10-6、8×10-6、16×10-6的再矿化液(实验组)中进行再矿化处理3周,SEM观察处理后人工釉质龋脱矿釉质表面的再矿化效果。结果不同浓度含硅再矿化液都有促进脱矿釉质再矿化的作用,其中以含硅质量分数为8×10-6的再矿化液效果最为显著。当含硅质量分数为0.5×10-6、1×10-6和8×10-6时,随着硅含量的增加脱矿牙釉质的再矿化程度增高;当含硅质量分数上升为16×10-6时,其再矿化程度则有所下降。结论硅能够促进脱矿牙釉质再矿化,并且以再矿化液含硅质量分数为8×10-6的再矿化效果最为显著。

ObjectiveTo observe the effect of different concentration of silicon on demineralized enamel,and investigate its effect on remineralization of enamel demineralization.MethodsThe research was performed in laboratory of School of Stomatology,Jilin University from April to August 2014.Enamel specimens were placed in lactic acid gel for one week.Then all specimens were randomly divided into 5 groups after we confirmed that artificial enamel caries were formed by SEM.Then the five groups of demineralized model were respectively placed in the remineralization solutions whose concentration of silicon was 0,0.5 × 10-6,1 × 10-6,8 × 10-6and 16 × 10-6.All specimens were removed after three weeks.The remineralization effect of the enamel surface were evaluated by scanning electron microscopy（SEM）.Results Different concentration of silicon had an effect on promoting remineralization of demineralized enamel,especially when the concentration of silicon was 8 × 10-6.With the increase of silicon concentration,the remineralization of enamel demineralization increased when the concentration of silicon was 0.5 × 10-6,1 × 10-6and 8 × 10-6.When the concentration of silicon increased to 16 × 10-6,its remineralization decreased.ConclusionSilicon has an effect on promoting remineralization of demineralized enamel,and the remineralization effect is the best when the concentration of silicon is 8 ×10-6.