大红袍中一个新的香豆素和八个已知酚酸类化合物

A new coumarin and eight known polyphenolics from Radix Campylotropidis Hirtellae

目的研究大红袍（RCH）的化学成分。方法利用重结晶、硅胶、ODS、Sephadex LH-20等色谱技术对大红袍60%乙醇提取物进行分离并纯化得到化合物,采用现代波谱技术阐明化合物结构。结果分离得到9个化合物,其中,1个新化合物1的结构根据波谱解析结果予以确定并命名为红花岩黄芪香豆雌酚Ⅰ（1）,而8个已知化合物则分别鉴定为异槲皮酚（isotrifoliol,2）、红花岩黄芪香豆雌酚B（3）、伞形花内酯（4）、香豌豆酚（5）、槲皮素（6）、（＋）儿茶素（7）、山奈酚（8）和二氢山奈酚（9）。结论化合物1为新化合物,化合物3~5首次从该植物中分离得到。抗H5N1病毒细胞活性筛选结果显示,化合物5在50.0μg/ml水平对H5N1有一定的抑制活性。

Objective To investigate the chemical constituents of the Radix Campylotropidis Hirtellae（ RCH）.Methods 60% ethanol extract of the RCH was separated by column chromatography on silica gel,ODS and Sephadex LH-20 to obtain aimed compounds. Structures of the compounds were investigated by modern spectroscopy. Results Nine compounds were separated from Campylotropis hirtella（ Franch.） Schindl,and the structure of the new compound 1 was determined by spectral analysis and named hedysarimcoumestan Ⅰ（ 1）,while the others were identified as the known isotrifoliol（ 2）,hedysarimcoumestan B（ 3）,umbelliferone（ 4）,orobol（ 5）,quercetin（ 6）,（ ＋） catechin（ 7）,kaempferol（ 8） and dihydrokaempferol（ 9）. Conclusion Compound 1 is a new compound and 3- 5 are separated from C. hirtella for the first time. The screening results of inhibition of H5N1 virus activity show that compound 5 has some inhibitory activity at a level of 50. 0 μg / ml.