油红O染色在大鼠脊髓损伤中的应用

Application of oil red O staining in spinal cord injury of rats

目的 ：探讨油红O染色在评价大鼠脊髓损伤中的应用价值。方法：选取体重220~260 g的SD雄鼠24只,编号后简单随机抽样分为正常对照组与脊髓横断组,正常对照组6只,脊髓横断组18只。脊髓横断组于T10节段横断脊髓。于术后1、2、4周在脊髓横断组分别随机各抽取6只处死,以横断部位为中心取脊髓组织标本。正常组取相同部位脊髓组织标本,行冰冻切片,进行油红O染色观察。结果：正常对照组大鼠脊髓白质均匀着色,与灰质有较明显的区分。脊髓横断组在术后1、2、4周时白质着色不均匀,断端液化坏死灶呈进行性扩大,油红O染色逐渐明显。结论：油红O染色能够较明显的区分白质及灰质,在一定程度上反映了白质的完整程度。断端坏死灶内油红O染色随脊髓损伤时间的延长逐渐明显反映了脂质在脊髓损伤过程中起着重要作用。

Objective：To explore the value of the application of oil red O staining in spinal cord injury（SCI） of rats.Methods：With simple randomization,24 Spargue-Dawley male rats were divided into normal control group including 6,and SCI group including 18. Spinal cord was transected at spinal lever T10 to build SCI model. Six rats of SCI group were sacrificed randomly at 1,2,4 weeks after surgery. After the spinal cord tissue sections were made,oil red O staining methods were used to observe the changes at the end of transected spinal cord. Images were analyzed by Image-Pro Plus 6.0 and SPSS 20.0 software.Results：The oil red O staining of normal control group showed that white matter surrounded by myelin sheath was clear and obviously distinctive from grey matter. Uneven and strengthened staining in oil O was observed in grey matter of SCI group at1,2,4 weeks post-SCI. Conclusion：It is a good method to label the myelin sheath in spinal cord and distinct white matter from grey matter by oil red O staining. Analysis of the images showed that lipid may become another target for drugs,which needs more researches.