地骨皮醇提液对胰岛β细胞增殖和凋亡的影响

Effect and Mechanism of Cortex Lycii Radicis Extracts on the Proliferation and Apoptosis of Pancreatic Beta Cell

[目的]以大鼠INS-1胰岛素瘤细胞株作为研究对象,用中药地骨皮(Cortex Lycii)醇提液干预INS-1细胞株,观察不同浓度地骨皮醇提液对胰岛β细胞增殖、凋亡变化的影响,为中药保护胰岛β细胞提供实验室依据。[方法]将INS-1细胞株分为正常糖对照组(control)、高糖组(high glucose,HG)、HG+Cortex Lycii(1g·L-1)组、HG+Cortex Lycii(2g·L-1)组以及HG+Cortex Lycii(4g·L-1)组,分别于药物干预24h、48h、72h后通过CCK-8法检测各组INS-1细胞的增殖率、采用流式细胞术检测细胞的凋亡率,并行相应统计学分析。[结果]48h、72h时与HG组相比,HG+Cortex Lycii各浓度组INS-1细胞的增殖率均有提高(P<0.01);与另外两个药物浓度组相比,72h时HG+Cortex Lycii(2g·L-1)组细胞增殖率最高,差异有统计学意义(P<0.05)。与HG组相比,HG+Cortex Lycii(1g·L-1)组、HG+Cortex Lycii(2g·L-1)组和HG+Cortex Lycii(4g·L-1)组INS-1细胞凋亡率显著下降,差异有统计学意义(P<0.01);HG+Cortex Lycii(2g·L-1)组凋亡率低于HG+Cortex Lycii(1g·L-1)组,差异有统计学意义(P<0.05)。[结论]地骨皮醇提液可促进高糖环境下INS-1细胞的增殖,抑制高糖环境下INS-1细胞的凋亡,其最佳的药物浓度为2g·L-1。

[Objective] Observing Cortex Lycii Radicis effect（Cortex Lycii） on rat insulinoma cells（INS-I） proliferation and apoptosis. To explore the mechanism of Cortex Lycii on Pancreatic Beta Cell Proliferation and apoptosis.[ Methods] After primary culture, cells were randomly divided into blank control group ：control,11.1mmol·L^-1 glucose, HG, 30mmol· L^-1 glucose, HG ＋ Cortex Lycii（lg· L^-1）, HG ＋ Cortex Lycii（2g·L^-1）, HG ＋ Cortex Lycii（4g· L^-l）, the survival rate of cells was observed by cell counting kit-8（CCK-8）; the apoptosis rate was observed by Annexin V stammg. [Results] 1 Compared with HG the better effect of cell proliferation groups of Cortex Lycii（P〈0.01）, the best is group of Cortex Lycii（2g·L^-1）（P〈0.05） ~）Compared with HG group the higher survival rate is group of Cortex Lycii（P〈0.01）, the lower apoptosis rate of Cortex Lycii（2g·L^-1） compared with Cortex Lycii（lg·L^-1）. [Conclusion] Cortex Lycii can promote the proliferation of pancreatic beta cell, inhibit the apoptosis to protect the pancreatic beta cell. The optimal concentration is 2g· L^-1.