HIV+患者氧化锆全冠修复后龈沟液IL-17的检测分析

Investigation on the levels of interleukin-17 in gingival crevicular fluid from HIV+ patients with zirconia ceramic crowns

目的:研究HIV+患者氧化锆全冠修复对龈沟液IL-17的水平影响。方法:16名HIV+接受氧化锆全冠修复的患者作为受试者,检测修复体数目为21颗,以对侧同名天然牙作为对照组。用吸潮纸尖吸取龈沟液。采用ELISA方法检测龈沟液中IL-17的水平,记录相关临床指标和血液CD4+T淋巴细胞计数,并分析其相关性。结果:HIV+患者氧化锆全冠修复后龈沟液IL-17浓度比对照组略高(P<0.05),而IL-17总量以及牙周指数和对照组没有明显区别(P>0.05),但两组的IL-17水平和牙周指数均与CD4计数有关(P<0.05)。结论:氧化锆全冠修复并没有增加HIV+患者牙周破坏程度,天然牙和氧化锆全冠修复后牙齿的牙周情况均与CD4计数即全身免疫状况有关。

Objective： To investigate the levels of interleukin-17（IL-17） in gingival crevicular fluid（GCF） samples from teeth with zirconia ceramic crowns and natural teeth and explore the correlation between the levels of IL-17 and periodontal clinical indices and CD4＋ T cell count in HIV＋ patients. Methods： The GCF samples were collected using paper strips in21 teeth with zirconia ceramic crowns and 21 natural teeth controls from 16 HIV＋ patients. After sampling, the periodontal indices were recorded. The CD4＋ T cells from blood sample were counted by flow cytometry. The levels of IL-17 in GCF samples were detected by ELISA assay. The relationship between them was analyzed. Results： The concentration of IL-17 in crown group was slightly higher than that in the control group total（P〈0.05）, but the total amount of IL-17 and periodontal indices have no significant difference compared with control group（P〉0.05）. The periodontal indices and the levels of GCF IL-17 in two groups were positively correlated with CD4^＋ T cell count（P〈0.05）. Conclusion： Zirconia ceramic crown restoration does not increase the risk of periodontal destruction in HIV＋ patients, and the periodontal condition of natural teeth and zirconia crown restoration teeth are correlated with CD4^＋ T cell count and systemic immune status.