紫外-可见分光光度法测定八爪金龙中总皂苷的含量

Determination of the Content of Total Saponins in Ardisia Crispa by Ultraviolet Spectrophotometry

目的：测定苗药八爪金龙中总皂苷的含量。方法：用紫外-可见分光光度法进行含量测定,采用正交试验优化测定条件。结果：该法测定的最优条件为加入5%香草醛-冰醋酸0.6 ml和高氯酸1.0 ml,于80℃水浴中反应20 min;线性方程为Y=44.87X＋0.365（r=0.998）,百两金皂苷B在0.045 2-3.870 0 mg范围内呈良好线性关系;平均回收率为101.3%（n=9）,RSD为1.67%。结论：该法用于测定八爪金龙中总皂苷的成分,具有简单、易操作、重复性良好的优点,可为进一步研究八爪金龙提供参考。

OBJECTIVE： To determine the total saponins content in Ardisia Crispa. METHODS： The total saponins content in Ardisia Crispa was determined by ultraviolet spectrophotometry. Orthogonal experiment was used to optimize the determination condition. RESULTS： The optimized conditions were as follows： adding 5% vanillic-acetic acid（ 0. 6 ml） and perchloric acid（ 1 ml） for water bath at 80 ℃ for 20 min. The linear relation was Y = 44. 87 X ＋ 0. 365（ r = 0. 998）. The linear range of Ardisiacrispin B was 0. 045 2-3. 870 0 mg. The averaged recovery rate was 101. 3%（ n = 9） with RSD = 1. 67%. CONCLUSIONS： The method used is simple and reproducible and it serves as references for the stuty of Ardisia Crispa.