石榴鞣花酸对高脂血仓鼠肝脏脂质合成与分解基因表达的影响

EFFECT OF POMEGRANATE ELLAGIC ACID(PEA) ON THE EXPRESSION OF LIPOGENESIS AND LIPOLYSIS GENES IN HYPERLIPIDEMIC HAMSTERS

目的观察石榴鞣花酸(PEA)对饮食诱导性高脂仓鼠脂质合成与分解相关基因的影响,探讨PEA降脂的作用机制。方法采用HPLC法测定PEA主要成分;54只雄性仓鼠随机均分为6组:正常对照组(NG,8w普通饲料);五个高脂组(HFG):模型对照组(MG)、药物对照组(辛伐他汀1.77mg/kg bw,SG)、PEA低(PEA-L 44 mg/kg bw)、中(PEA-M 88 mg/kg bw)、高(PEA-H 177 mg/kg bw;)剂量组,后5组为前4w高脂饲料,后4w普通饲料;4w高脂模型建立成功后,以灌胃方式连续给予仓鼠对应处理,第4w、8w检测仓鼠血清TC、TG含量;采用RT-PCR法测定肝脏中脂质代谢调控因子固醇调节元件结合蛋白-1c(SREBP-1c)m RNA表达和脂肪酸合成酶(FAS)以及脂蛋白脂肪酶(LPL)m RNA表达量。结论 PEA可以降低血液中TC、TG的含量,升高HDL-C的含量,主要通过抑制SREBP-1c m RNA、FAS m RNA的基因表达,促进LPL m RNA的基因表达实现降脂功能。PEA降脂效果存在剂量依赖关系,高剂量PEA效果较好。

Objective The study was aimed to further investigate the effects of pomegranates ellagic acid （PEA） on lipid metabolism and relevant gene expressions in hyperlipidemic hamster.Method 54 male hamsters were randomly divided into six groups of equal size： normal group （NG）, model group （MG）, simvastatin group （SG） with 1.77mg/kg simvastatin, PEA low group （PEA-L） with PEA 44 mg/kg, PEA medium group （PEA-M） with PEA 88 mg/kg, PEA high group （PEA-H） with PEA 177 mg/kg. The last 4 groups were treated with simvastatin or PEA for 4 weeks after 4 weeks, respectively. Except NG was given normal diet, other 5 groups were fed a high fat diet at the first 4 weeks and switched to normal diet for the next 4 weeks. At the end of weeks 4 and 8, plasma total cholesterol （TC）, triglyceride （TG） and high-density lipoprotein cholesterol （HDL-C） were detected by kits. Real time PCR was used to analyze the mRNA expression of adipogenesis genes sterol regulatory element-binding protein-1c （SREBP-1c）, fatty acid synthase （FAS） and lipoprotein lipase （LPL）.Results PEA reduced plasma TC and TG and increased HDL-C, accompanied by down-regulation mRNA expression of SREBP-1c, FAS and up-regulation mRNA expression of LPL.Conclusion PEA had a dose-dependent effect and was comparable with simvastatin.