摘要
从海南乐东豇豆地采集到的枯萎病病样,经形态学观察、致病性测定和真菌ITS序列分析,将其病原鉴定为尖孢镰刀菌菜豆专化型（Fusarium oxysporum f.sp.phaseolus）。生物学特性分析表明：该病原菌菌丝生长适宜温度为20-30℃,最适温度为25℃,50℃时不能生长;适宜pH为6-10,最适pH值为8.0;全黑暗和全光照有利于菌丝的生长;可利用多种碳、氮源,最适碳源为果糖和D-甘露醇,氮源为蛋白胨和硝酸钠。
Samples of cowpea fusarium wilt were collected from a cowpea field in Ledong, Hainan province. The pathogens were identified as Fusarium oxysporum f. sp. phaseolus by the methods of morphological characters,pathogenicity testing in vivo and sequence analysis of fungic ITS. At the same time, biological characteristic analysis showed that the temperature of the mycelium growth was range from 20℃ to 30℃. The optimum temperature was 25℃. This strain could not grow when the temperature was up to 50℃. The pH value of mycelium growth was range from 6 to 10. The optimal pH value was 8.0. It was grown best under full lighting and complete darkness. It can utilize many kinds of carbon and nitrogen sources. And the optimum carbon sources were fructose and D-mannitol, the optimum nitrogen sources were peptone and sodium nitrate.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2015年第2期345-349,共5页
Genomics and Applied Biology
基金
公益性行业(农业)科研专项资金(201303023)
十二五国家科技支撑计划(2012BAD19B06)
海南省农科院农业科技创新专项(CXZX201416)
国家星火科技计划项目(NO.2012GA800026)共同资助
关键词
豇豆枯萎病
病原鉴定
系统进化分析
生物学特性
Cowpea fusarium wilt
Pathogenic identification
Phylogenetic analysis
Biological characteristic
