丹参转录因子SmMYB7的克隆及表达模式分析

Cloning and Expression Analysis of the Transcription Factor SmMYB7 from Salvia miltiorrhiza Bunge

分析丹参转录组数据库(SRX021907),得到一条R2R3-MYB基因,blast比对发现该基因为丹参Sm MYB7(KF059361.1)。分别从g DNA和c DNA水平克隆该基因全长,测序结果表明该基因与公布序列一致,分析发现该基因无内含子序列,包含一个长为954 bp的开放读码框(ORF),编码317个氨基酸残基。多重序列比对和系统进化树分析显示Sm MYB7蛋白与拟南芥At MYB73同属R2R3-MYB第22个亚家族。已有丹参基因信息结合BD walking获得1 974 bp的启动子序列,分析结果表明多种顺式作用元件存在于该基因的启动子区。实时荧光定量PCR分析显示,该基因的表达为组成型,在丹参根、茎、叶、花中都表达;随着花的发育,该基因的表达量逐渐增加;此外,Sm MYB7在盐胁迫、水杨酸、茉莉酸甲酯和脱落酸处理下表达均上调,推测该基因参与调控植物防御和花的发育。

A transcription factor named SmMYB7（KF059361.1） of R2R3-MYBfamilywas found by blastsearching in NCBI and transcription database（SRX021907） of Salvia miltiorrhiza Bunge. Its full-length of cDNA and DNA sequence was cloned. Sequencing results of the gene what we cloned was consistent with the one in NCBI. SmMYB7 contains one open reading frame with 954 bp, which encods 317 amino acids. Through blast searching in survey database of S. miltiorrhiza and BD walking, we got the promoter region which exists lots of cis-acting elements and is composed by 1 974 nucleotides. According to the results of real-time quantitative PCR, it expressed in all tissues.With the development of flower, the expression level of SmMYB7 is enhanced. Besides, its expression was up-regulated by salt, salicylic（SA）, jasmonate（Me JA） and abscisic acid（ABA）, which indicates that SmMYB7 maybe play a role in defenses and flower development.