IL-10对重症急性胰腺炎大鼠血清IL-2、IL-8及胰腺组织的影响

Effects of IL-10 on serum IL-2,IL-8 and pancreas in rats with severe acute pancreatitis

目的探讨大鼠血清中IL-2、IL-8及IL-10等细胞因子在重症急性胰腺炎(SAP)中的作用,为临床治疗SAP提供相关理论依据。方法 Wistar大鼠120只,分为三组。SAP组:6%的L-arginine诱导大鼠SAP;IL-10组:造模后腹腔内注射IL-10;C组:各时间点给予生理盐水作为正常对照。分批处死大鼠,ELISA检测血清IL-2、IL-8、IL-10的水平,并对胰腺组织进行组织病理学评分。结果 IL-10组血清IL-2、IL-8水平及胰腺病理评分在多个时间点显著高于C组并且低于SAP组(P<0.05),而IL-10显著高于C组和SAP组(P<0.05)。结论 (1)血清IL-2、IL-8是促炎性细胞因子,与SAP的病变程度正相关;IL-10是抗炎性细胞因子,与SAP的病变程度负相关。(2)IL-10可作为潜代药物应用于急性胰腺炎的治疗。

Objective To investigate the effects of interleukin-2,interleukin-8 and interleukin-10 of serum in severe acute pancreatitis（ SAP）,to provide a new approach for clinical treatment and theory basis for SAP. Methods 120 wistar rats were randomly divided into three groups. Group SAP received 6% L-arginine to induce SAP model; Group IL-10 also received L-arginine and injection IL-10 after the last injection of L-arginine.Group C only received physiological saline. Then killed the rats,serum IL-2,IL-8 and IL-10 were assayed by ELISA,and pancreatic tissues were fixed in formalin. Histopathological score was recorded in three groups.Results The level of IL-2,IL-8 and histopathology scores were higher than group C and lower than group SAP several times（ P〈0. 05）,however,IL-10 was higher than group C and group SAP（ P〈0. 05）. Conclusions IL-2 and IL-8 are able to promote the severity of SAP,however,IL-10 have the opposite effect. Maybe IL-10 can be a potential drug for the clinical treatment of acute pancreatitis.