黄芩苷对DSS诱导结肠炎小鼠Th22细胞及IL-22的影响

Effect of Baicalin on Th22 and IL-22 in DSS-induced Colitis Mice

目的:观察黄芩苷对炎症性肠病小鼠模型Th22细胞比例及IL-22浓度的影响,通过体内和体外实验探讨黄芩苷治疗该病的免疫学机制。方法 :C57BL/6小鼠用3.5%葡聚糖硫酸钠(dextran sodium sulfate)建立结肠炎小鼠模型,随机分成空白对照组、模型组、黄芩苷组。应用流式细胞术和酶联免疫吸附试验(ELISA)分别检测各组小鼠Th22细胞比例和外周血血清IL-22的表达;分离小鼠脾脏淋巴细胞,用含黄芩苷(0、10、20、40μmol·L-1)培养基培养48 h,应用流式细胞术检测各组小鼠淋巴细胞中Th22细胞比例。结果:黄芩苷在体内外实验中均降低Th22细胞比例与IL-22的表达。结论:黄芩苷能够在体外和DSS诱导的结肠炎小鼠体内抑制Th22的分化及IL-22的表达,提示黄芩苷对Th22介导的炎症性疾病具有较好的治疗潜力。

This study was aimed to investigate the effect of baicalin on the proportion of Th22 cells and theconcentration of IL-22 both in vivo and in vitro, in order to explore the immune mechanism of baicalin on inflammatory bowel disease mice model. The 3.5% dextran sodium sulfate（DSS） was used on C57BL/6 mice for the establishment of colitis mice model. Mice were randomly divided into the blank control group, model group, and baicalin group. Flow cytometry and ELISA were used in the detection of the proportion of Th22 cells and concentration of IL-22 in peripheral blood serum, respectively. The spleen lymphocytes of mice were isolated and cultured by baicalin medium（0, 10, 20, 40 μM） for 48 h. Flow cytometry was used in the detection of the proportion of Th22 icne lvlisv. oThe results showed that baicalin reduced the proportion of Th22 cells and the expression of IL-22 both and in vitroin vitro experiments. It was concluded that baicalin can inhibit Th22 cell differentiation and expression of IL-22 and DSS-induced colitis mice. It indicated that baicalin had a good treatment potential in Th22 cell-mediated inflammatory diseases.