新型CDK抑制剂联合ZD55-MnSOD溶瘤腺病毒体外抑制人结肠癌细胞增殖的研究

In-Vitro Inhibitory Effect of Oncolytic Adenovirus ZD55-MnSOD Combined with New CDK Inhibitor on Human Colon Cancer Cells

探究携带MnSOD的溶瘤腺病毒Ad-E1B55Kd-MnSOD(ZD55-MnSOD)联合小分子药物新型CDK抑制剂SNS-032对人结肠癌细胞株SW620、HCT116、HT-29生长的体外抑制效果,通过溶瘤病毒与药物联合,以期寻求较好的抗癌作用。实验采用MTT法检测5 MOI的ZD55-MnSOD与不同剂量的SNS-032(0、20、40、80、160ng/mL)联合处理人结肠癌细胞株,发现病毒与药物联合处理有促进肿瘤细胞凋亡的作用。ZD55-MnSOD(5 MOI)与SNS-032(80ng/mL)联合处理SW620、HT-29细胞,发现联合作用具有很大程度上的时间依赖性,Hoechst33342染色法和流式细胞仪检测细胞凋亡也显示ZD55-MnSOD与SNS-032联合处理组的细胞凋亡现象比二者单独使用均具有显著差异。该研究初步证实了ZD55-MnSOD与SNS-032联合具有互补作用,能有效地在体外抑制人结肠癌细胞的增殖。

The research is to develop in-vitro inhibitory effect of MnSOD carried oncolytic adenovirus Ad-E1B55Kd-MnSOD（ZD55-MnSOD）combiend with small molecular drug novel CDK inhibitor SNS-032 on colon cancer cell lines such as SW620,HCT116 and HT-29.Oncolytic virus and drugs were combined to seek good antitumor effects.In this experiment,MTT was used to detect the application effect of 5MOI ZD55-MnSOD combined with different dosage of SNS-032（0,20,40,80,160ng/mL）on human colon cell line.The authors found that the combination of oncolytic virus and drug could promot tumor cell apoptosis;and also found that treatment of SW620 and HT-29 with combined ZD55-MnSOD（5MOI）and SNS-032（80ng/mL）had great time dependence.Hoechst33342 staining method and flow cytometry were used to detect apoptosis.The results aslo show that the apoptosis phenomenon in the group of combined use of ZD55-MnSOD with SNS-032 has better effects than separate use of oncolytic virus or drug alone.This research preliminarily proves ZD55-MnSOD（5MOI）and SNS-032 have supplementary effect and can effectively inhibit the growth of colon cancer cells in vitro.