细胞外基质蛋白1与原发性肝内胆管细胞癌及淋巴结转移的相关性分析

Correlation of extracellular matrix protein 1 with primary intrahepatic cholangiocarcinoma and ;lymph node metastasis

目的：研究细胞外基质蛋白1（ECM1）在原发性肝内胆管细胞癌（PICC）发生及淋巴结转移（LNM）中的应用价值。方法 PICC无转移组9例、PICC有LNM组21例，正常胆管组织标本10例。行免疫组织化学（IHC）染色和评估、RT-PCR mRNA实验。结果 IHC染色显示：ECM1在正常胆管组织标本不表达，PICC中以及LNM标本中均有表达。ECM1 mRNA在正常胆管组织标本中很少，在PICC中异常升高，LNM的患者中升高更多，差异有统计学意义（均为P〈0.001）。ECM1 mRNA与PICC未转移的相关系数r=0.979，P=0.000。ECM1 mRNA与PICC有LNM的相关系数r=0.871，P=0.000。Logistic回归分析表明，ECM1是PICC的独立预测指标，公式为总分=7.992log（ECM1＋0.001）-5.200，分界点为0.00，得分为0.00以上时，被视为恶性。结论 ECM1与PICC发生及LNM有良好的相关性。

Objective To investigate the application value of extracellular matrix protein 1 （ECM1） in primary intrahepatic cholangiocarcinoma （PICC） and lymph node metastasis （LNM）. Method There were 3 groups in the study,including PICC without metastasis（n=9）, PICC with LNM metastasis（n=21）,and normal bile duct tissue specimens（n=10）. Immunohistochemical（IHC）staining and evaluation,and RT-PCR of mRNA were used in the study. Results IHC staining showed that no expression of ECM1 was found in normal bile duct tissue specimens,whereas expression of ECM1 was found in both PICC specimens with and without LNM metastasis. The expression level of ECM1 mRNA in normal bile duct tissue specimens was low,but abnormally increased in non-metastatic PICC patients and further increased in PICC with LNM metastasis（all P〈0.001）. The correlation coefficient between ECM1 mRNA and PICC without metastasis was 0.979（P=0.000）. The correlation coefficient between ECM1 mRNA and PICC with LNM was 0.871 （P=0.000）. Logistic regression analysis showed that ECM1 was an independent predictor of PICC,based on the formula：total score=7.992 log（ECM1＋0.001）-5.200. The cut-off point was 0.00. The score above 0.00 would be indicative of malignancy. Conclusion ECM1 shows good correlation with the occurrence of PICC and LNM metastasis.