71例自身免疫性肝病自身抗体检测回顾性分析

A retrospective analysis of the detection of autoantibodies of 71 cases of autoimmune liver disease

目的探讨自身免疫性肝病自身抗体的检测及临床应用价值。方法采用间接免疫荧光法、免疫印迹法检测自身免疫性肝病(AIH、PBC、PSC)患者血清中出现的ANA、ASMA、AMA抗体,并对AMA-M2亚型、抗SLP/LP、抗LKM-1、抗LC-1肝病自身抗体进行检测。结果自身免疫性肝病患者抗核抗体的阳性率为61.97%,抗体的滴度水平>1∶100,在抗核抗体的荧光模型中,AIH主要以颗粒型、均质型为主,PBC主要以核膜型、着丝点型为主。在AIH中,抗SLP/LP、抗LKM-1、抗LC-1、AMA-M2的检出率分别为16.67%、12.50%、4.17%、4.17%。结论利用对自身免疫性肝病自身抗体的检测,有助于帮助诊断和鉴别不明原因的肝脏疾病,提高对自身免疫性肝病的诊断和治疗。

Objective To investigate the detection and clinical application value of autoantibodies in autoimmune liver disease.Methods Using indirect immunofluorescence and immunoblotting to detect ANA,ASMA,AMA antibody in serum of patients with autoimmune hepatitis（ AIH,PBC and PSC） and autoantibodies of the subtype AMA- M2,anti SLP / LP,anti LKM- 1,anti LC- 1 were detected. Results The anti nuclear antibodies in patients with autoimmune liver disease positive rate was61. 97%,and the antibody titer levels was above 1∶100; in the fluorescence model of anti nuclear antibody,AIH mainly was in particle type,homogeneous type; PBC was mainly in the nuclear membrane,centromere type. In AIH,the detection rate of anti SLP / LP,anti LKM- 1,anti LC- 1 and AMA- M2 was respectively 16. 67%,12. 50%,4. 17%,4. 17%. Conclusion Dectecting the autoantibodies in autoimmune liver disease can help to diagnose,identify the cause of liver disease,improve the diagnosis and treatment of autoimmune liver disease.