利用D-半乳糖诱导小鼠评价罗仙子初提物的抗衰老药效

Anti-aging effect of luoxianzi extract on D-gal-induced aging mice

目的研究罗仙子初提物对D-半乳糖(D-gal)诱导小鼠的抗衰老药效。方法昆明小鼠48只,随机分为正常对照组、衰老模型组、罗仙子初提物高、中、低剂量组和维生素E(Vit E)组。除正常对照组外,其余各组均按10 mg/kg给予D-gal颈背部皮下注射,每日1次,连续注射6周,同时罗仙子初提物和Vit E组按10 m L/kg灌胃给药。在实验开始第30天,进行避暗实验;并于6周后处死小鼠,检测小鼠血清、肝脏和脑组织中各项抗氧化相关指标。结果与正常对照组相比,衰老模型组小鼠体质量增加速率明显减慢,学习记忆能力明显减退,小鼠血清、肝脏、脑组织超氧化物歧化酶(SOD)活性和谷胱甘肽过氧化物酶(GSH-PX)活力降低,丙二醛(MDA)含量增加。与衰老模型组相比,Vit E组和罗仙子初提物各组体质量增加速率升高,学习记忆能力增强,小鼠血清、肝脏、脑组织SOD活性升高,MDA含量减少,GSH-PX活力增强。结论罗仙子初提物对D-gal诱导小鼠有抗衰老作用,其作用机制可能与提高小鼠体内SOD活性,减少MDA含量以及提高GSH-PX活力等有关。

Objective To study the anti-aging effect of luoxianzi extract on D-galactose（ D-gal）-induced aging mice. Methods KM mice were randomly divided into the normal control group,the D-gal-induced model group,three dosages of luoxianzi extract groups and the vitamin E group. The aging model was established by subcutaneous injection in cervicodorsal region with D-gal（ 10 mg / kg） once a day for six weeks. Meantime,luoxianzi extract and vitamin E were given by intragastric administration. The effect of luoxianzi extract on learning and memory ability of mice was observed. After 6 weeks,the superoxide dismutase（ SOD） activity,malondialdehyde（ MDA） content and telomerase activity in sera,liver and brain tissues of mice were measured. Results Compared with the normal control group,the weight increase declined,the number of errors in step-down test increased,and the activities of SOD and GSH-PX in serum,liver and brain tissues dropped and the MDA content was raised in aging mice. Compared with the model group,the weight increase boosted,the activities of SOD and GSH-PX in sera,liver and brain tissues enhanced and the MDA content reduced in mice treated with luoxianzi extract or vitamin E. Conclusion Luoxianzi extract exhibits anti-aging effect on Dgal-induced aging mice,by which mechanism is related to up-regulation of SOD and GSH-PX activities and decrease of MDA content in serum,liver and brain of aging mice.