宫颈癌患者血清Th1及Th2细胞因子的表达水平分析

Analysis on expression levels of serum Th1/Th2 cytokines in cervical cancer patients

目的探讨不同临床分期宫颈癌患者血清Th1和Th2细胞因子水平与Th1、Th2细胞因子表达相关关系。方法选择72例宫颈癌患者作为病例组,并进行FIGO临床分期,同时选择30例健康体检女性作为对照组,Elisa法检测干扰素-γ(IFN-γ)、白介素-2(IL-2)等Th1细胞因子水平和白介素-4(IL-4)、白介素-6(IL-6)等Th2细胞因子水平,并计算Th1/Th2比值。结果病例组患者血清IFN-γ、IL-2、IL-4和IL-6水平高于对照组,而IFN-γ/IL-4、IFN-γ/IL-6、IL-2/IL-4和IL-2/IL-6比值低于对照组,差异有统计学意义(P<0.05);随着宫颈癌临床分期的加重,血清Th1和Th2细胞因子水平逐渐增高,而Th1/Th2细胞因子比值则逐渐降低,差异均有统计学意义(P<0.05);病例组患者血清IFN-γ与IL-4、IL-6分别呈明显正相关(r=0.533,0.502),血清IL-2与IL-4、IL-6也分别呈明显正相关(r=0.606,0.574),差异均有统计学意义(P<0.05)。结论宫颈癌患者Th2细胞因子水平异常增高,Th1/Th2细胞因子动态平衡发生紊乱,并且二者互相影响。

Objective To explore the expression levels of serum Th1/ Th2 cytokines in cervical cancer patients of different clinical stages and the relationship between serum Th1/Th2 cytokines .Methods Totally 72 cervical cancer patients at different clinical stages based on FIGO classification were selected as case group and another 30 healthy women were chosen as control group. Serum Thl cytokines （IFN- γ, IL- 2） and Th2 cytokines （IL- 4, IL- 6） were determined by ELISA, and the ratio of Th1/Th2 was calculated. Results The levels of serum IFN-γ, IL-2, IL-4 and IL-6 in patients of case group were significandy higher than that of control group, whereas the ratio of IFN-γ/IL-4, IFN-γ/IL-6, IL-2/IL-4 and IL-2/IL-6 in patients of case group were significantly lower than that of control group （P〈0.05）. With the deterioration of the cervical cancer, the levels of serum Thl cytokines and Th2 cytokines showed a rising trend significantly, while the ratio of Th1/Th2 cytokines significantly decreased （P〈0.05）. In case group, the levels of serum IFN-γ were significantly positive correlated with IL-4 and IL-6 （r=0.533 , 0.502, respectively）, and the levels of serum IL-2 were significantly positive correlated with IL-4 and IL- 6 （r=0.606 , 0.574 respectively, P〈0.05）. Conclusions The levels of serum Th2 eytokines in cervical cancer patients elevated significantly. The dynamic balance of serum Th1/Th2 cytokines was disturbed resulting in interaction of Th1 and Th2 cytokines